Purpose This observational study aimed to evaluate the stability of unilateral posterior crossbite (UPXB) correction with the functional appliance function generating bite (FGB appliance) during follow-up and its effects on craniofacial growth. Materials and method A total of 102 age- and gender-matched patients were included: 51 with UPXB (male [M] = 19; female [F] = 32; mean age +/- standard deviation [SD] = 7.6 +/- 1.4 [years.months]) and 51 controls (C; M = 19; F = 32; mean age +/- SD = 7.9 +/- 1.3). UPXB was corrected with the FGB appliance. Study casts were collected before treatment (T0), after correction (T1), after a follow-up of 3.7 +/- 1.6 [years. months] (T2) and different transversal measurements were performed with calipers: intermolar (IMD), intermolar gingival (IMGD), intercanine (ICD), and intercanine gingival distances (ICGD). In treated patients, in addition, lateral cephalometric tracings were analyzed at T0 and T2. Results At T0, all maxillary measurements were significantly smaller in the UPXB group compared to the controls (p < 0.001). Between T0 and T1, all maxillary variables increased significantly in the UPXB group (p < 0.001; IMD +4.3 +/- 2.1 mm; ICD +3.1 +/- 2 mm). Between T1 and T2, maxillary IMD increased further (p < 0.05; +2.2 +/- 2 mm). The mean T0-T2 increases were 6.6 +/- 2.6 mm (IMD) and 4.3 +/- 2.6 mm (ICD). At T0, the cephalometric analysis showed significantly stronger mandibular clockwise rotation (p < 0.01) in the UPXB group compared to the control group. Between T0 and T2, the UPXB group showed a significant reduction of mandibular (p < 0.001; SpP-CoOr T0 = -3.47 degrees +/- 4.38, T2 = -1.14 degrees +/- 4.01) and occlusal plane (p < 0.01; SpP-Oc T0 = 11.37 degrees +/- 3.91, T2 = 9.86 degrees +/- 3.83) clockwise rotation, and of intermaxillary divergence (p < 0.01; SpP-GoGn T0 = 27.8 degrees +/- 5.39, T2 = 26.65 degrees +/- 5.49). Conclusions Treatment of UPXB with the FGB appliance effectively increased maxillary transversal width measurements, especially in the posterior region, even after follow-up, indicating stable results. Concurrently, mandibular clockwise rotation was reduced, indicating control of the vertical dimension.