Dual-functional improvement of vascularization and bone formation of complementary PRP-based gel scaffold

At present, it is found that the ability of vascular formation will directly determine the final repair effect When repairing the bone defect. Platelet-rich plasma (PRP) is extensively applied in bone repair because its various growth factors, but the explosive release of PRP growth factors affects the repair effect of bone defect in the later stage, and simvastatin can promote bone repair through local recruitment of growth factors.Therefore, we constructed a simvastatin-loaded PRP gel composite titanium alloy microporous dual-functional scaffold system to achieve better bone repair results by locally recruiting growth factors from simvastatin in the later stage. The experiments exhibited that the system had good biocompatibility, and human umbilical vein endothelial cells (HUVECs) exhibited good migration ability. ALP staining and quantitative analysis exhibited that bone marrow mesenchymal stem cells (BMSCs) exhibited higher. In vivo, this strategy was found to achieve higher newbone formation and angiogenesis of lateral femoral bone defects model by Micro-CT and its analysis and tissue immunofluorescence staining. Theoretical evidence is provided in this study that constructing a bioactive interface to promote local blood vessels and bone regeneration, and provides a new treatment idea for orthopedic treatment of large-area bone defects.