Objective: Automatic emergency braking systems with pedestrian detection (PAEB) are effective at preventing pedestrian crashes, but the safety benefits are not observed at night. This study used the Insurance Institute for Highway Safety (IIHS) PAEB test data to characterize PAEB responses in different lighting conditions and for different rated systems. Methods: Data from 6,919 IIHS PAEB tests were retrieved from IIHS databases. Invalid trials, trials without AEB, and trials with outliers were removed leaving 5,894 trials from 212 model year 2018 to 2023 vehicles for analysis. PAEB responses were characterized by computing the time-to-collision (TTC) of forward collision warning (FCW) and AEB; brake threat number (BTN); mean deceleration; maximum deceleration; and maximum jerk. A linear mixed-effects model was used to predict each dependent measure with scenario (crossing adult, stationary adult), speed, rating (superior, basic/advanced), lighting (day, night with high beams, night with low beams), and their interactions. Vehicle was included as a random effect. A Bonferroni correction was applied to maintain a family-wise type-1 error rate of 0.05 across 138 total hypothesis tests. Results: PAEB system warnings were later and automatic braking occurred later as speed increased at night with low beams but changed little at night with high beams and during the day (p < 0.0004). BTN increased more rapidly as speed increased at night with low beams compared with high beams and during the day (p < 0.0004). Based on the BTN model, on average, PAEB systems can brake to avoid the adult mannequin (BTN < 1) when closing speed is less than 67 km/h during the day and at night with high beams but only when closing speed is less than 49 km/h at night with low beams. Superior-rated PAEB systems warned and braked earlier compared with basic/advanced-rated systems (p < 0.0004). Conclusions: Increased lighting from high beams made nighttime performance resemble daytime performance in controlled testing. Increasing output from vehicle low beams, increasing the use of high beams, and enhancing overhead lighting around crosswalks and pedestrian areas are all methods for increasing lighting, improving pedestrian conspicuity, and enhancing PAEB performance to prevent pedestrian crashes.