The Influence of Catechin on Adiponectin Gene Expression and Insulin Resistance in Obese Rats

OBESITY represented as an abnormal accumulation of visceral fat, which is closely related with insulin resistance, adipokine expression. The generation of adiponectin (AdipoQ) has been linked to visceral fat accumulation. Obesity, insulin resistance, and cardiovascular disease have all been linked to decreased levels of AdipoQ. Fifteen white female rats were used in the experiment; they were divided into three primary groups equally as negative control, positive control and Cat-fed group. After 28 days of restricted diet for negative control group the weight became 150g, while the weight of the positive control group with high fat diet change to 217g significantly. First group and positive control group killed after the 28 days from starting day. While, the five remaining rats treated with (+) Catechin for another 28 days as Catechin treated group then killed for getting samples. The blood and visceral adipose tissue samples from all groups used to measure glucose, insulin and lipid profile, and AdipoQ. Treatment with (+) Catechin increased the levels of AdipoQ in visceral WAT as well as improved fasting glucose and insulin levels resulting in amelioration of Insulin Resistance in Cat-fed group compared to positive control group. A significant difference of circulating glucose, insulin, and AdipoQ levels and gene expression inside visceral WAT obtained between means of positive control with Catechin treatment group.