Urine and serum-based ELISA using a recombinant protein and synthetic peptide for the diagnosis of tegumentary leishmaniasis

被引：0

作者：

Freitas, Camila S. ^{[1
]}

Camara, Raquel S. B. ^{[1
]}

Lage, Daniela P. ^{[1
]}

Vale, Danniele L. ^{[1
]}

Silva, Ana L. ^{[1
]}

Pimenta, Breno L. ^{[1
]}

Ludolf, Fernanda ^{[1
,2
]}

Galvani, Nathalia C. ^{[1
]}

de Jesus, Marcelo M. ^{[1
]}

Assis, Barbara P. N. ^{[1
,3
]}

Chaves, Ana T. ^{[1
]}

Tavares, Grasiele S. V. ^{[1
]}

Tupinambas, Unai ^{[1
]}

Chavez-Fumagalli, Miguel A. ^{[4
]}

Pascoal, Vanessa P. M. ^{[5
]}

Eller, Marcela T. C. ^{[6
]}

Rocha, Manoel O. da Costa ^{[1
]}

Christodoulides, Myron ^{[7
]}

Machado-de-Avila, Ricardo A. ^{[8
]}

Goncalves, Denise U. ^{[1
]}

Pereira, Isabela A. G. ^{[1
]}

Coelho, Eduardo A. F. ^{[1
,9
]}

机构：

[1] Univ Fed Minas Gerais, Fac Med, Programa Posgrad Ciencias Saude Infectol & Med Tro, BR-30130100 Belo Horizonte, MG, Brazil

[2] Fac Ciencias Med Minas Gerais, Programa Posgrad Ciencias Saude, BR-30130110 Belo Horizonte, MG, Brazil

[3] Hosp Eduardo Menezes, Fundacao Hosp Estado Minas Gerais, BR-30622020 Belo Horizonte, MG, Brazil

[4] Univ Catolica Santa Maria, Urb San Jose S N, Arequipa, Peru

[5] Fiocruz MS, Fundacao Oswaldo Cruz, Ctr Pesquisas Rene Rachou, BR-30190009 Belo Horizonte, MG, Brazil

[6] Policlin Municipal Doutor Roberto Shuffner, BR-39803001 Teofilo Otoni, MG, Brazil

[7] Univ Southampton, Southampton Gen Hosp, Sch Clin & Expt Sci, Neisseria Res Grp,Fac Med,Mol Microbiol, Southampton, England

[8] Univ Extremo Catarinense, Programa Posgrad Ciencias Saude, BR-88806000 Criciuma, SC, Brazil

[9] Univ Fed Minas Gerais, Dept Patol Clin, COLTEC, BR-31270901 Belo Horizonte, MG, Brazil

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2025年 / 111卷 / 03期

关键词：

Tegumentary leishmaniasis; Recombinant protein; ELISA; Diagnosis; Urine; Serum; VISCERAL LEISHMANIASIS; ANTIGEN; SERODIAGNOSIS;

D O I：

10.1016/j.diagmicrobio.2024.116631

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The diagnosis of tegumentary leishmaniasis (TL) presents problems by the variable sensitivity and specificity of the tests, and the biological samples used are also invasive. Here, ELISA experiments were performed using paired TL patient urine and serum samples in reaction against the recombinant LiHyS protein, a predicted B cell epitope and parasite antigenic extract (SLA). Two hundred and five paired samples were used, which were provided by TL patients, healthy controls and patients with Chagas disease, leprosy, malaria or HIV-infected. An urine-based ELISA showed sensitivity values of 100%, 92.1%, and 82.5%, when rLiHyS, peptide and SLA were used, respectively; and specificity of 100%, 87.6%, and 79.5%, respectively. A serum-based ELISA showed sensitivity values of 100%, 99.3%, and 81.5%, using rLiHyS, peptide and SLA, respectively, and sensitivity of 100%, 96.5%, and 72.2%, respectively. In both cases, rLiHyS presented the better performance to diagnose TL by using patient serum and urine.

引用

页数：8

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