Structural characteristics of polysaccharide isolated from Potentilla anserina L. and its mitigating effect on Zearalenone-induced oxidative stress in Sertoli cells

被引:1
|
作者
Shi, Haixia [1 ]
Yan, Zunqiang [1 ]
Du, Hong [1 ]
Song, Kelin [1 ]
Gun, Shuangbao [1 ,2 ]
机构
[1] Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730030, Peoples R China
[2] Gansu Innovat Res Ctr Swine Prod Engn & Technol, Lanzhou 730070, Peoples R China
关键词
Potentilla anserina L; Polysaccharide; Structure characterization; Sertoli cell; Antioxidant activity; IN-VITRO; FRUITING BODIES; ANTIOXIDANT; VIVO;
D O I
10.1016/j.ijbiomac.2025.139752
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study aims to characterize the structural features of a natural polysaccharide called PAP-1b extracted from the roots of Potentilla anserina L. and to evaluate its antioxidant activity. Structural characterization indicated that PAP-1b with a molecular weight of 1.22 x 104 Da was primarily composed of glucose and galactose. Methylation and NMR analyses showed that PAP-1b mainly consisted of -> 4)-alpha-D-Glc p-(1 ->, -> 4,6)- (3- D-Glc p- (1 ->, -> 3,4)-alpha-Glc p-(1 -> and alpha-D-Glcp-(1 ->). Subsequently, we evaluated the antioxidant activity of PAP-1b using zearalenone (ZEA)-induced oxidative stress in porcine Sertoli cells (SCs) as a model. Cellular experiments revealed that PAP-1b significantly attenuated ZEA-induced oxidative stress in SCs via the mitochondrial pathway, as evidenced by the increase in cell viability, the enhancement of antioxidant enzyme activities, and the reduction of reactive oxygen species (ROS), lactate dehydrogenase (LDH) and malondialdehyde (MDA) levels, as well as stabilization of the mitochondrial membrane potential and the reduction of apoptosis rate. These results suggest that Potentilla anserina L. polysaccharides can serve as a promising natural antioxidant for applications in the field of functional foods.
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页数:13
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