Tracking of Antibiotic Resistance Genes in Sludge from Treatment Plants for Organic Fraction of Municipal Solid Waste

被引:0
|
作者
Franchitti, Elena [1 ]
Mafrica, Antonino [1 ]
Traversi, Deborah [1 ]
机构
[1] Univ Turin, Dept Publ Hlth & Pediat, Piazza Polonia 92, I-10126 Turin, Italy
关键词
antimicrobial resistance; antibiotic resistance genes; ddPCR; anaerobic digestion; organic fraction of municipal solid waste; waste management; circular economy; TETRACYCLINE RESISTANCE; SULFONAMIDE; VALIDATION; PRIMERS; COLI; PCR;
D O I
10.3390/su162410922
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Bacteria quickly acquire the ability to survive or grow in the presence of an antibacterial agent that should be able to inhibit or kill them, leading to increased mortality caused by infective diseases. The digestate from the anaerobic digestion (AD) of the organic fraction of municipal solid waste (OFMSW) is spread on soil, but the knowledge on the presence and persistence of the antibiotic resistance genes (ARGs) is limited. Thus, this study aims to evaluate the presence of seven ARGs (blaTEM, blaOXA, ermB, qnrB, sulI, sulII, tetA, and tetW) in the OFMSW and their persistence after the AD, using the innovative droplet digital Polymerase Chain Reaction (ddPCR), not yet used on this matrix. A total of 31 samples were collected from the influent and effluent of the AD in two plants located in Northern Italy. ARG concentration ranged between 4 and 9 Log gene copies/kg of sludge. A limited reduction in the concentration of antibiotic resistance targets given by AD was observed in the study (<1 Log). The persistence of ARGs after mesophilic digestion of the OFMSW suggests a risk of horizontal transmission when directly spread on soil. Further evaluations are needed for safe and sustainable reuse of such sludges.
引用
收藏
页数:11
相关论文
共 50 条
  • [1] BIOGAS FROM ORGANIC FRACTION OF THE MUNICIPAL SOLID-WASTE AND PRIMARY SLUDGE
    CECCHI, F
    TRAVERSO, P
    CHIMICA & L INDUSTRIA, 1986, 68 (09): : S9 - S13
  • [2] Compost characteristics from sewage sludge and organic fraction of municipal solid waste
    Zorpas, AA
    Stamatis, V
    Zorpas, GA
    Vlyssides, AG
    Loizidou, M
    FRESENIUS ENVIRONMENTAL BULLETIN, 1999, 8 (3-4): : 154 - 162
  • [3] Effects of Activated Carbon on the Fate of Antibiotic Resistance Genes During Anaerobic Digestion of the Organic Fraction of Municipal Solid Waste
    Ma J.-Y.
    Wang P.-L.
    Wang B.-H.
    Su Y.-L.
    Xie B.
    Huanjing Kexue/Environmental Science, 2021, 42 (05): : 2413 - 2421
  • [4] Dynamics of Antibiotic Resistance Genes During the Municipal Solid Waste Leachate Treatment
    Huang F.-Y.
    Zhou S.-Y.-D.
    Yan Y.-J.
    Su J.-Q.
    Zhu Y.-G.
    Zhang X.
    Huanjing Kexue/Environmental Science, 2019, 40 (10): : 4685 - 4690
  • [5] Rheology of sludge from double phase anaerobic digestion of organic fraction of municipal solid waste
    Battistoni, P
    Pavan, P
    Mata-Alvarez, J
    Prisciandaro, M
    Cecchi, F
    WATER SCIENCE AND TECHNOLOGY, 2000, 41 (03) : 51 - 59
  • [6] Co-digestion of the organic fraction of municipal solid waste with primary sludge at a municipal wastewater treatment plant in Turkey
    Dereli, Recep Kaan
    Ersahin, Mustafa Evren
    Gomec, Cigdem Yangin
    Ozturk, Izzet
    Ozdemir, Ozgur
    WASTE MANAGEMENT & RESEARCH, 2010, 28 (05) : 404 - 410
  • [7] Co-digestion Approaches to Organic Fraction of Municipal Solid Waste with Primary Sludge for a Municipal Treatment Plant in Turkey
    Dereli, R. K.
    Ersahin, M. E.
    Gomec, C. Y.
    Ozdemir, O.
    Ozturk, Izzet
    SURVIVAL AND SUSTAINABILITY: ENVIRONMENTAL CONCERNS IN THE 21ST CENTURY, 2011, : 1005 - 1014
  • [8] Release of airborne antibiotic resistance genes from municipal solid waste transfer stations
    Fan Lü
    Wei Wang
    Tianyu Hu
    Haowen Duan
    Liming Shao
    Hua Zhang
    Pinjing He
    Sustainable Environment Research, 32
  • [9] Release of airborne antibiotic resistance genes from municipal solid waste transfer stations
    Lu, Fan
    Wang, Wei
    Hu, Tianyu
    Duan, Haowen
    Shao, Liming
    Zhang, Hua
    He, Pinjing
    SUSTAINABLE ENVIRONMENT RESEARCH, 2022, 32 (01)
  • [10] Pilot-Scale Polyhydroxyalkanoate Production from Combined Treatment of Organic Fraction of Municipal Solid Waste and Sewage Sludge
    Valentino, Francesco
    Moretto, Giulia
    Lorini, Laura
    Bolzonella, David
    Pavan, Paolo
    Majone, Mauro
    INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2019, 58 (27) : 12149 - 12158