Background: Antimicrobial resistance (AMR) is a major global public health concern, particularly in low- and middle-incomecountries where resources and infrastructure for an adequate response are limited. The World Health Organization (WHO) GlobalAntimicrobial Resistance Surveillance System (GLASS) was introduced in 2016 to address these challenges, outliningrecommendations for priority pathogen-antibiotic combinations. Despite this initiative, implementation in Africa remainsunderstudied. This scoping review aims to assess the current state of implementing WHO GLASS recommendations on antimicrobialsensitivity testing (AST) in Africa.Objective: The primary objective of this study is to determine the current state of implementing the WHO GLASSrecommendations on AST for priority pathogen-antimicrobial combinations. The review will further document if the reportingof AST results is according to "susceptible," "intermediate," and "resistant" recommendations according to GLASS.Methods: Following the methodological framework by Arksey and O'Malley, studies published between January 2016 andNovember 2023 will be included. Search strategies will target electronic databases, including MEDLINE, Scopus, CINAHL, andEmbase. Eligible studies will document isolates tested for antimicrobial sensitivity, focusing on WHO-priority specimens andpathogens. Data extraction will focus on key study characteristics, study context, population, and adherence to WHO GLASSrecommendations on AST. Descriptive statistics involving summarizing the quantitative data extracted through measures ofcentral tendency and variation will be used. Covidence and Microsoft Excel software will be used. This study will systematicallyidentify, collate, and analyze relevant studies and data sources based on clear inclusion criteria to provide a clear picture of theprogress achieved in the implementation of the WHO GLASS recommendations. Areas for further improvement will be documentedto inform future efforts to strengthen GLASS implementation for enhanced AMR surveillance in Africa.Results: The study results are expected in August 2024.Conclusions: To our knowledge, this scoping review will be the first to comprehensively examine the implementation of WHOGLASS recommendations in Africa, shedding light on the challenges and successes of AMR surveillance in the region. Addressingthese issues aims to contribute to global efforts to combat AMR.International Registered Report Identifier (IRRID): PRR1-10.2196/58140
