Strategic improvement of Shewanella oneidensis for biocatalysis: Approach to media refinement and scalable application in a microbial electrochemical system

被引：0

作者：

Juergensen, Nikolai ^{[1
]}

Weiler, Janek R. ^{[1
]}

Knoll, Melanie T. ^{[1
]}

Gescher, Johannes ^{[1
]}

Edel, Miriam ^{[1
]}

机构：

[1] Hamburg Univ Technol, Inst Tech Microbiol, D-21073 Hamburg, Germany

来源：

MATERIALS LETTERS | 2025年 / 382卷

关键词：

Shewanella oneidensis; Bioelectrochemical system; Microbial electrolysis cell; Current density; Scale-up; ELECTRON-TRANSFER; YEAST EXTRACT; CHEMICALS; BIOFILM; FLAVINS; MR-1;

D O I：

10.1016/j.nbt.2024.11.006

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Microbial electrochemical systems offer a sustainable method for the conversion of chemical energy into electrical energy or hydrogen and the production of valuable compounds, contributing to the development of a biobased economy. This study aimed to enhance the performance of anodic bioelectrochemical systems by improving the current density of Shewanella oneidensis as a biocatalyst through strain modification and medium refinement. The genetic modification, combining the prophage deletion and overexpression of the speC gene, resulted in a 4.2-fold increase in current density compared to the wild type. Furthermore, medium refinement and incorporating riboflavin, led to an additional 5.7-fold increase in current density. The application of the modified strain and medium in a scalable microbial electrolysis cell resulted in a current density of 1.2 A m-2, similar to what was achieved previously with an S. oneidensis and Geobacter sulfurreducens co-culture, substantiating the substantial performance increase for a pure culture of S. oneidensis. Furthermore, S. oneidensis was shown to grow in medium containing up to 500 mM sodium chloride and increasing the salt concentration to 400 mM had a minor influence on growth but significantly lowered the cell voltage of the MEC system.

引用

页码：31 / 38

页数：8