Long-Term Monitoring of ApoE4 within Aqueous Humor Using Intraocular Lenses Containing Target-Responsive Inverse Opal Hydrogel

被引：0

作者：

Lee, Hyo ^{[1
]}

Lim, Jaewook ^{[1
]}

Shin, Moo-Kwang ^{[1
]}

Moon, Chae-Eun ^{[2
]}

Lee, Jun-Ki ^{[2
]}

Kim, Jiwon ^{[1
]}

Kang, Yujin ^{[2
]}

Ji, Yong Woo ^{[2
,3
]}

Haam, Seungjoo ^{[1
]}

机构：

[1] Yonsei Univ, Dept Chem & Biomol Engn, Seoul 03722, South Korea

[2] Yonsei Univ, Coll Med, Inst Vis Res, Dept Ophthalmol, Seoul 03722, South Korea

[3] Yonsei Univ, Yongin Severance Hosp, Dept Ophthalmol, Coll Med, Seoul 16995, Gyeonggi Do, South Korea

来源：

SMALL STRUCTURES | 2024年 / 5卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

Alzheimer's disease; aqueous humor; fluorescence amplification; intraocular lens; inverse opal hydrogels; ALZHEIMERS-DISEASE; APOLIPOPROTEIN-E; BIOMARKERS; MICROSPHERES; DIFFUSION; EYES;

D O I：

10.1002/sstr.202400154

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Neurodegenerative diseases, including Alzheimer's disease (AD), pose significant challenges to global healthcare because of their irreversible postsymptomatic progression. Conventional radiographic techniques have limitations in early detection, owing to the time lapse between symptom recognition by individuals and precise inspection using medical instruments. Current research explores ophthalmic approaches to address this gap, investigating the physiological link between ocular health and brain diseases. Intraocular lenses (IOLs), widely used in cataract and refractive surgeries, provide a safe and minimally invasive platform for the continuous monitoring of neurodegenerative disease biomarkers, including those associated with AD. By integrating biomolecule-responsive sensors with IOLs, it becomes feasible to achieve early diagnosis and long-term, noninvasive monitoring postimplantation. Herein, a fluorescently labeled inverse opal hydrogel (FIOH) is merged with IOLs for ApoE4 detection, a key risk factor for AD. The permeability and inherent photonic properties of FIOH facilitate the cumulative enhancement of the fluorescence signal. In a simulation of the aqueous humor circulation, the detection limit for ApoE4 is determined to be 0.1 nM. Therefore, FIOH-integrated IOLs hold promise not only for early detection but also for providing a reliable platform for the continuous, noninvasive monitoring of neurodegenerative diseases after the initial, minimally invasive implantation.

引用

页数：13