Biodegradation and bioaugmentation of the co-contamination of chloramphenicol and microplastics by Exiguobacterium sp. CAP4 isolated from a contaminated plastisphere

Microplastics (MPs) and antibiotics are newly emerging contaminants that have heavily accumulated in the environment and are a great cause of concern due to their co-contamination. Although the removal and degradation of individual MPs and antibiotics have been studied in various environments, our understanding of how to eliminate the co-contamination of MPs and antibiotics remains poor. In this study, the biodegradation of both micro polyethylene (mPE) and chloramphenicol (CAP) was analyzed in a wastewater sample. Members of the genera Exiguobacterium, Methanospirillum, Methanosaeta, and Candidatus Nitrocosmicus were proposed as biomarkers in plastisphere, which may contribute to the biodegradation of both contaminants. Notably, Exiguobacterium sp. CAP4 was isolated from the plastisphere and exhibited a high potential to degrade both CAP and mPE. Bioaugmentation with Exiguobacterium sp. CAP4 in mPEs and CAP contaminated wastewater facilitated the biodegradation of both mPE and CAP. This work expands the knowledge base regarding the simultaneous elimination of MPs and antibiotics in situ and identifies a promising bacterial strain for both MP and antibiotic biodegradation.