The genetic impact of pomegranate extract and chitosan nanoparticles on the swarming motility of Proteus mirabilis

Aims: After Escherichia coli and Klebsiella pneumoniae, Proteus mirabilis is the third most common cause of urinary tract infections. This study aims to investigate the genetic influence of two genes ( flaA and wosA) and quantify them using the RT-qPCR technique on the phenomenon of swarming motility in P. mirabilis. Methodology and results: The swarmer cells were treated with two natural inhibitors, a hot aqueous extract of pomegranate peels and chitosan nanoparticles. flaA and wosA were genetically investigated in different culture media and treated with natural inhibitors using the RT-qPCR technology. Normal swarm cells had considerably more flaA and wosA genes than vegetative cells. Their amounts decreased when the agar dropped to 0.75% and when the NaCl was 1%. The impact of chitosan nanoparticles outweighed that of pomegranate peel hot aqueous extract. Conclusion, significance and impact of study: The appropriate medium for producing typical swarm cells of agar is 1.5%. The natural inhibitors had a substantial and effective on the P. mirabilis infection. Its inhibition prevents bacteria from migrating on the surface of the catheter, so inhibition catheter-associated urinary tract infection (CAUTIS).