Ontogeny of Thyroid Hormone Signaling in the Retina of Zebrafish: Effects of Thyroidal Status on Retinal Morphology, Cell Survival, and Color Preference

被引：0

作者：

Lazcano, Ivan ^{[1
]}

Pech-Pool, Santiago M. ^{[1
]}

Maldonado-Lira, Maria Fernanda ^{[2
]}

Olvera, Aurora ^{[1
]}

Darras, Veerle M. ^{[3
]}

Orozco, Aurea ^{[1
,4
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Neurobiol, Campus Juriquilla,Blvd Juriquilla 3001, Queretaro 76230, Mexico

[2] Univ Autonoma Queretaro, Fac Quim, Ctr Univ, Queretaro 76010, Mexico

[3] Katholieke Univ Leuven, Lab Comparat Endocrinol, Biol Dept, B-3000 Leuven, Belgium

[4] Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Campus Juriquilla, Queretaro 76230, Mexico

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 22期

关键词：

thyroid hormones; retina development; zebrafish; deiodinases; thyroid hormone receptor; TYPE-3; DEIODINASE; GENE-EXPRESSION; EYE DEVELOPMENT; MECHANISMS; VISION; RECEPTORS; BRAIN; LEVEL;

D O I：

10.3390/ijms252212215

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The retina is crucial for converting light into neuronal signals for visual perception. Understanding the retina's structure, function, and development is essential for vision research. It is known that the thyroid hormone (TH) receptor type beta 2 (TR beta 2) is a key element in the regulation of cone differentiation in the retina, but other elements of TH signaling, such as transporters and enzyme deiodinases, have also been implicated in retinal cell development and survival. In the present study, we investigated the expression profile of genes involved in TH signaling and analyzed the impact of thyroidal status on retinal morphology, opsin expression, cell death/proliferation profile, as well as color preference behavior during the early retina development of zebrafish larvae. mRNA expression analysis on dissected whole eyes revealed that TH signaling elements gradually increase during eye development, with dio3b being the component that shows the most dramatic change. Mutations generated by CRISPR/CAS9 in the dio3b gene, but not in the thrb gene, modifies the structure of the retina. Disruption in TH level reduces the cell number of the ganglion cell layer, increases cell death, and modifies color preference, emphasizing the critical importance of precise TH regulation by its signaling elements for optimal retinal development and function.

引用

页数：18