Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia

被引:0
|
作者
Malewa, Amirudin Dg [1 ]
Rusdin, Rusdin [1 ]
Mangun, Mardiah [1 ]
Khaerunnisa, Isyana [2 ]
Lestari, Dwi [2 ]
Duma, Yulius [1 ]
机构
[1] Tadulako Univ, Fac Anim Sci & Fisheries, Dept Anim Husb, Palu 94119, Indonesia
[2] Natl Res & Innovat Agcy, Res Ctr Appl Zool, Bogor 16911, Indonesia
关键词
IGF-1; GH; morphometric; Napu buffalo; polymorphism; GROWTH-HORMONE GENE; SHORT COMMUNICATION; BIRTH-WEIGHT; CATTLE; ASSOCIATION;
D O I
10.5455/javar.2025.l887
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Objective: The objectives of this study are to measure the body weight (BW) and morphometric parameters of Napu buffaloes, identify the growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene polymorphisms, and associate them with the BW and morphometric parameters of Napu buffaloes. Materials and Methods: This study used 39 Napu buffaloes (9 males and 30 females). Morphometric measurements were performed using a measuring tape. The GH and IGF-1 gene diversity analysis was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques. Results: The results showed that there is diversity in BW and body size of Napu buffaloes due to the influence of age and sex. The results of PCR-RFLP analysis of the GH|AluI gene showed 0% VV, 100% LL, and 0% VL genotypes. While the IGF-1|SnaBI gene shows the genotype AA 0%, BB 100%, and AB 0%. The sequencing results of the GH and IGF-1 genes in Napu buffaloes did not find mutations. Conclusion: Age and sex in Napu buffaloes may affect BW and body size. The GH and IGF-1 genes in Napu buffaloes are monomorphic, so no association can be made with the morphometric parameters of Napu buffaloes.
引用
收藏
页码:205 / 213
页数:9
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