Green Synthesis of Silver Nanocomposite from Zataria Multiflora Boiss Extract for Antioxidant, Antibacterial and Anticancer Activities

Silver nanocomposites have received considerable interest in medical application. In the present work, silver nanoparticles/ordered mesoporous carbon (AgNPs/OMC) nanocomposite has been synthesized using Zataria multiflora Boiss extract as a natural reducing and stabilizing agent. The FE-SEM images showed rod-shape AgNPS with the main particle size of 33.6 +/- 1.4 nm which is in agreement with XRD results (AgNPS with 41.83 nm and face-central cubic structure on OMC). The average surface concentration (Gamma) of the Ag onto OMC was obtained (1.07 +/- 0.36) x 10(-10) mol/cm(2).The AgNPs/OMC exhibited excellent antioxidant, antibacterial and anticancer activity. The content of total phenols 12 ppm and DPPH free radical scavenging 81.23% were calculated. The antibacterial behavior of AgNPs/OMC nanocomposite was investigated on Escherichia coli and Staphylococcus Aureus bacterial species. The results exhibited strong antibacterial activity against bacterial species especially Staphylococcus aurous with minimum inhibition concentration (MIC, 31.25 mu g/mL) and minimum bactericidal concentration (MBC, 62.5 mu g/mL). Also, in this work, we evaluated cell cytotoxicity effects of AgNPs/OMC against breast cancer (AU145). The cell test experiments (MTT) showed higher viability loss and significant toxicity of AgNPs/OMC nanocomposite (300 mu g/mL) against AU145 with cell viability of similar to 23 % and similar to 12% at 24 and 48, respectively. The half-maximal inhibitory concentration (IC50) value was found significantly low with AgNPs/OMC (in comparison to OMC) because of the presence of rich AgNPs on OMC. Thus, AgNPs/OMC nanocomposite due to unique physical and chemical properties, large surface area and higher scavenging properties could be considered as potential medical agent, which may be applied in clinical systems.