Introduction: The quality of life of professors is an important standard for their job performance. Objective: To determine the influence of workload on the quality of life of professors at a Peruvian university. Materials and Methods: This was a study with a quantitative, explanatory, cross-sectional approach applied to 326 active employees and professors at a Peruvian university during the years 2023 and 2024. Two questionnaires validated in Peru were administered. The questionnaires asked about sociodemographic data, SF-36 quality of life, and the workload scale. The data were analyzed using Studio R software, correlation analysis with Pearson R, and the explanatory model with linear regression methods. Results: It was found that, in the workload, an average of 11.56 was obtained, which shows a workload with low distribution among professors. Discussion: It was found that there is a positive and moderate correlation between workload and four dimensions of quality of life, which are physical role, bodily pain, emotional role, and social functioning; it was observed that these are significant predictors of workload. Conclusion: It is concluded that workload and quality of life influence each other. However, no relationship was found between the variables; important specific correlations were identified between workload and three dimensions of quality of life (emotional role, bodily pain, and social functioning).