Systematic molecular dissection of key intermediates in xanthones biosynthesis in Cudrania tricuspidata and establishment of a viable heterologous expression system

被引：0

作者：

Zhang, Jing ^{[1
]}

Xu, Dan-Dan ^{[1
]}

Zhu, Ting-Ting ^{[1
]}

Ta, He ^{[1
]}

Hao, Yue ^{[1
]}

Zhang, Jiao-Zhen ^{[1
]}

Fu, Jie ^{[1
]}

Cheng, Ai-Xia ^{[1
]}

Lou, Hong-Xiang ^{[1
]}

机构：

[1] Shandong Univ, Cheeloo Coll Med, Sch Pharmaceut Sci, Key Lab Chem Biol,Minist Educ, Jinan 250012, Shandong, Peoples R China

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2025年 / 220卷

基金：

中国国家自然科学基金;

关键词：

Benzophenone 3 ' -hydroxylase; benzophenone synthase; Benzoyl-coenzyme A ligase; Cudrania tricuspidata; Heterogenous biosynthesis; Xanthone derivatives; BENZOPHENONE SYNTHASE; CHALCONE SYNTHASE; NATURAL-PRODUCTS; CELL-CULTURES; COENZYME; BENZOATE; LIGASE; PURIFICATION; INSIGHTS;

D O I：

10.1016/j.plaphy.2025.109524

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The major phytochemicals in the roots of Cudrania tricuspidata are prenylated xanthones, exhibiting significant structural diversity and bioactive properties, such as anti-inflammatory, antioxidative, and antitumor effects. The biosynthetic pathways of these compounds have not yet been resolved, limiting their production through synthetic biology. In this study, benzoyl-coenzyme A (CoA) ligase (BZL), benzophenone synthase (BPS), and benzophenone 3 '-hydroxylase (B3 ' H) transcripts involved in the biosynthesis of xanthone were cloned and characterized from C. tricuspidata. The results showed that C. tricuspidata BZL (CtBZL) catalyzed the formation of benzoyl-CoA from benzoate and C. tricuspidata BPS (CtBPS) catalyzed the condensation of benzoyl-CoA and three molecules of malonyl-CoA to form 2,4,6-trihydroxybenzophenone (2,4,6-triHB) via Claisen type cyclization. Then, C. tricuspidata B3 ' H (CtB3 ' H) hydroxylated 2,4,6-triHB to produce 2,3 ',4,6-tetrahydroxybenzophenone (2,3 ',4,6-tetraHB), which was a key precursor of xanthone derivatives. In addition, the functions of the enzymes were further explored by the heterologous expression of CtBZL, CtBPS, and CtB3 ' H in Saccharomyces cerevisiae and Nicotiana benthamiana. Also, a preliminary heterologous synthesis system of 2,3 ',4,6-tetraHB was established. The present study provided the candidate genes and strategies for the heterologous production of xanthones using S. cerevisiae and N. benthamiana as hosts.

引用

页数：10