Extrachromosomal DNA in Breast Cancer Cell Lines: Detection and Characterization

被引：0

作者：

Irdianto, Shadira Anindieta ^{[1
]}

Fadhillah, Fadhillah ^{[1
]}

Lestari, Retno ^{[1
]}

Fadilah, Fadilah ^{[2
]}

Bowolaksono, Anom ^{[1
]}

Dwiranti, Astari ^{[1
]}

机构：

[1] Univ Indonesia, Fac Math & Nat Sci, Dept Biol, Cellular & Mol Mech Biol Syst CEMBIOS Res Grp, Depok, Indonesia

[2] Univ Indonesia, Fac Med, Dept Med Chem, Jakarta, Central Jakarta, Indonesia

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2024年

关键词：

breast cancer; extrachromosomal DNA; fluorescence imaging; MCF-7; MDA-MB-231;

D O I：

10.1002/jemt.24780

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

This study delves into the intriguing world of extrachromosomal DNA (ecDNA) in breast cancer, uncovering its pivotal role in cancer's aggressiveness and genetic variability. ecDNA, a form of circular DNA found outside chromosomes, is known to play a significant role in cancer progression by increasing oncogene expression. Focusing on two contrasting cell lines, MDA-MB-231 (triple-negative) and MCF-7 (Luminal-A), we utilized advanced microscopy and fluorescence techniques to detect and characterize ecDNA. Our findings reveal a stark difference: MDA-MB-231 cells, known for their high metastatic potential, exhibit a striking abundance of ecDNA, manifested as double minutes and single form with intense fluorescence signals. In contrast, the less aggressive MCF-7 cells harbor significantly fewer ecDNA. This disparity highlights the potential of ecDNA as a key player in cancer progression and a promising target for novel therapies. This research sheds light on the unseen genetic forces driving breast cancer and opens the door to new strategies in cancer treatment. Further research is necessary to understand the mechanisms of ecDNA formation and its role in different breast cancer subtypes.

引用

页码：1239 / 1247

页数：9