Influence of extraction method of Carica papaya seeds and Chrysanthemum roseum leaves on the acaricidal property against Rhipicephalus microplus

The present study aimed to assess the adulticidal and larvicidal efficacy of chloroform, methanol, and hexane extracts obtained from the Chrysanthemum roseum (leaves) and the Carica papaya (seeds) against Rhipicephalus microplus ticks. The percentage of extractability for all the extracts varied between 1.5% and 25%. The hexane extract of C. roseum showed a mortality rate ranging from 6.66 ± 6.66% to 100.00 ± 0.00% at doses ranging from 0.62 to 5%. An impact on the oviposition capacity of the treated ticks, resulting in a significant reduction of 90.15 ± 6.460% in oviposition at a concentration of 2.25% was also observed. The methanol extract of C. roseum exhibited a higher concentration of anti-tick action and did not demonstrate a significant influence on the reproductive potential of the treated ticks. A mortality rate of 33.33 ± 17.63 to 93.33 ± 6.67% for treated ticks during 24 h. of treatment, was observed at concentration range of 5 to 12.5% of hexane extract of Carica papaya, Furthermore, the extract also resulted in the suppression of egg-laying (98.72 ± 1.27%) at 12.5%. Against larvae, the extract exhibited equivalent efficacy and LC50 for LIT and LPT were determined as 2.7% and 0.08%, respectively.