Computer-Assisted Modeling and Simulation of a Dielectrophoresis-based Microseparator for Blood Cells Separation Applications

The objective of the present numerical investigation is to propose a microseparator specifically engineered for the separation of platelets from white blood cells (WBCs). This microfluidic device, which incorporates circular electrodes operating at a minimal voltage of 1.4 V, and a frequency of 100 kHz, is recommended for the targeted separation of platelets and WBCs utilizing dielectrophoresis (DEP) force. The utilization of a low-voltage level ensures the preservation of the viability of biological cells, a fundamental consideration in medical applications. Simulation results are presented to illustrate the electric potential, electric field, velocity, pressure, and DEP force profiles concerning two distinct particles. Through a comparative analysis employing the finite element method, we delineate the implications of modulating the inlet velocity field on the pressure exerted upon the particles. Subsequently, the impact of varying the fluid conductivity and input voltage on electrodes within the microchannel on particle separation was examined. It is anticipated that this comprehensive design is exceptionally conducive to the realization of DEP-based practical biochips for cell separation.