Unraveling the structural evolution of silver plasmonic hotspots for the detection of oxidative ONOO- radicals via SERS probe decay

被引：0

作者：

Zhu, Xiaoying ^{[1
,2
]}

Zhang, Qi ^{[2
]}

Qi, Xiaohua ^{[2
]}

Feng, Yibo ^{[3
]}

Zou, Mingqiang ^{[2
]}

Ma, Qingbian ^{[6
]}

Zhang, Lin ^{[5
]}

Ma, Qiang ^{[2
]}

Wang, Cong ^{[1
,4
]}

机构：

[1] Beijing Univ Technol, Fac Mat & Mfg, Beijing Key Lab Microstruct & Properties Solids, Beijing 100124, Peoples R China

[2] Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China

[3] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China

[4] Univ Chinese Acad Sci, Ningbo Inst Life & Hlth Ind, Ningbo 315012, Peoples R China

[5] State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China

[6] Peking Univ Third Hosp, Beijing 100191, Peoples R China

来源：

MICROCHIMICA ACTA | 2025年 / 192卷 / 03期

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

Ag nanomaterial; Structural evolution; SERS; ONOO-; radical; Biological sample; NANOPARTICLES; PEROXYNITRITE; EDGES;

D O I：

10.1007/s00604-025-07045-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Peroxynitrite (ONOO-) plays a pivotal role in environmental pollution and ecosystem health, necessitating its detection for assessing ecological impacts and risks. Surface-enhanced Raman scattering (SERS) offers high sensitivity but is often limited by narrow Raman cross sections of analytes. Specialized molecules can aid SERS detection, but are complex to design and may cause nonspecific reactions in biological systems. Therefore, developing new SERS strategies is crucial for simpler, more accurate ONOO- detection. Herein, the shape instability of Ag nanomaterials in the hotspots, due to oxidation and dissolution of Ag atoms at the edges and corners, is investigated, and the detection of ONOO- is performed by SERS probes. ONOO- reacts first with the (111) facet, especially at the edges and corners. Consequently, the SERS signal of the adsorbed probe, Rhodamine 6G in hotspots can be used to monitor edge and corner dissolution that positively related to the ONOO- concentration. As a result, ONOO- concentration from 0.1 to 25 mu M was detected, achieving a coefficient of determination of R-2 = 0.9896. The method exhibits good reproducibility (RSD < 3.25%) and stability (> 7 days), and quantitative detection of ONOO- was achieved in bovine serum samples. Ag nanocubes exhibited an eightfold stronger response and higher precision compared to Ag nanoparticles in ONOO- detection. This simple detection technique offers a promising method for the accurate, quantitative detection of ONOO- in wide range of biological systems.

引用

页数：13