A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-nuclei transcriptomics

被引：0

作者：

Segovia, Cristopher ^{[1
,2
]}

Desrosiers, Vincent ^{[3
,4
]}

Khadangi, Fatemeh ^{[1
,2
]}

Robitaille, Karine ^{[1
,2
,5
,6
]}

Armero, Victoria Saavedra ^{[7
]}

D'Astous, Myreille ^{[8
,9
]}

Khelifi, Gabriel ^{[1
,2
]}

Bergeron, Alain ^{[1
,2
,10
]}

Hussein, Samer ^{[1
,2
,11
]}

Richer, Maxime ^{[8
,9
]}

Bosse, Yohan ^{[7
,12
]}

Fradet, Yves ^{[1
,2
,8
,10
]}

Fradet, Vincent ^{[1
,2
,5
,6
,8
]}

Bilodeau, Steve ^{[1
,2
,11
,13
]}

机构：

[1] Univ Laval, Ctr Rech CHU Quebec, Axe Oncol, 1401,18e Rue, Quebec City, PQ G1J 1Z4, Canada

[2] Ctr Rech Canc Univ Laval, Quebec City, PQ G1R 3S3, Canada

[3] Univ Laval, Ctr Rech CHU Quebec, Axe Malad Infect & Immunitaires, Quebec City, PQ G1V 4G2, Canada

[4] Ctr Rech ARThrite Univ Laval, Quebec City, PQ G1V 4G2, Canada

[5] Univ Laval, Ctr Rech NUTRISS Nutr Sante & Soc, Quebec City, PQ G1V 4G2, Canada

[6] Inst Nutr & Aliments Fonct Univ Laval, Quebec City, PQ G1V 4G2, Canada

[7] Inst Univ Cardiol & Pneumol Quebec Univ Laval, Quebec City, PQ G1V 4G5, Canada

[8] Univ Laval, CHU Quebec, Quebec City, PQ G1R 2J6, Canada

[9] Univ Laval, Ctr Rech CHU Quebec, Axe Neurosci, Quebec City, PQ G1V 4G2, Canada

[10] Univ Laval, Fac Med, Dept Chirurg, Quebec City, PQ G1V 0A6, Canada

[11] Univ Laval, Fac Med, Dept Biol Mol Biochim Med & Pathol, Quebec City, PQ G1V 0A6, Canada

[12] Univ Laval, Fac Med, Dept Med Mol, Quebec City, PQ G1V 0A6, Canada

[13] Univ Laval, Ctr Rech Donnees Mass, Quebec City, PQ G1V 0A6, Canada

来源：

SCIENTIFIC REPORTS | 2025年 / 15卷 / 01期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

Nuclei isolation; Tissue heterogeneity; Transcriptomics; Cancer; Tissue homogenization; Nuclei sorting; CELL;

D O I：

10.1038/s41598-025-90070-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Clinical samples are vital for understanding diseases, but their scarcity requires refined research methods. Emerging single-cell technologies offer detailed views of tissue heterogeneity but need sufficient fully characterized tissues. We developed an optimized single-nuclei RNA sequencing (snRNA-seq) protocol to extract nuclei from just 15 mg of cryopreserved human tissue. Applied to four cancer tissues (brain, bladder, lung, prostate), it profiled 1550-7468 nuclei per tissue, revealing heterogeneity comparable to public single-cell atlases. This method enhances the use and sharing of rare, cryopreserved biospecimens, supporting research where sample quantity is limited and full tissue characterization is needed.

引用

页数：11

共 10 条

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Gordon Wiegleb
Susanne Reinhardt
Andreas Dahl
Nico Posnien
Frontiers in Zoology, 19
[2] Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material
Wiegleb, Gordon
Reinhardt, Susanne
Dahl, Andreas
Posnien, Nico
FRONTIERS IN ZOOLOGY, 2022, 19 (01)
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Xiaolu Zhang
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Nature Communications, 10
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Gebiola, Marco
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INSECT SCIENCE, 2022, 29 (05) : 1318 - 1328
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NEURO-ONCOLOGY, 2024, 26
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NATURE COMMUNICATIONS, 2021, 12 (01)
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Nature Communications, 12
[9] A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus
Conde, Daniel
Triozzi, Paolo M.
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PLOS ONE, 2021, 16 (05):
[10] An Efficient Method for Isolating and Purifying Nuclei from Mice Brain for Single-Molecule Imaging Using High-Speed Atomic Force Microscopy
Qiu, Yujia
Sajidah, Elma Sakinatus
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CELLS, 2024, 13 (03)

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