Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture

被引：0

作者：

Shi, Jingyi ^{[1
,2
]}

Wu, Mei ^{[1
]}

Fang, Shi ^{[1
]}

Liu, Zhuo ^{[1
]}

Liu, Huihui ^{[1
]}

Zhao, Ying ^{[2
]}

Liu, Linlin ^{[1
]}

Shao, Zhicheng ^{[1
]}

机构：

[1] Fudan Univ, Zhongshan Hosp, Inst Translat Brain Res,Dept Neurol, MOE Frontiers Ctr Brain Sci,State Key Lab Med Neur, Shanghai 200032, Peoples R China

[2] Xinxiang Med Univ, Sch Pharm, Xinxiang 453003, Peoples R China

来源：

CELL REGENERATION | 2025年 / 14卷 / 01期

关键词：

Saponins; Human embryonic stem cells; Culture medium; Brain organoid; PANAX-NOTOGINSENG SAPONINS; ADJUVANT; DERIVATION; DIFFERENTIATION; EXTRACTS; PATHWAY;

D O I：

10.1186/s13619-024-00220-y

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The cultivation and differentiation of human embryonic stem cells (hESCs) into organoids are crucial for advancing of new drug development and personalized cell therapies. Despite establishing of chemically defined hESC culture media over the past decade, these media's reliance on growth factors, which are costly and prone to degradation, poses a challenge for sustained and stable cell culture. Here, we introduce an hESC culture system(E6Bs) that facilitates the long-term, genetically stable expansion of hESCs, enabling cells to consistently sustain high levels of pluripotency markers, including NANOG, SOX2, TRA-1-60, and SSEA4, across extended periods. Moreover, organoids derived from hESCs using this medium were successfully established and expanded for at least one month, exhibiting differentiation into cortical organoids, GABAergic precursor organoids and heart-forming organoids. This innovative system offers a robust tool for preserving hESC homeostasis and modeling the nervous system in vitro.

引用

页数：13