Integration of bile proteomics and metabolomics analyses reveals novel insights into different types of gallstones in a high-altitude area

被引：0

作者：

Jing, Xiaofeng ^{[1
]}

Ma, Ying ^{[2
]}

Li, Defu ^{[2
]}

Zhang, Tiecheng ^{[1
]}

Xiang, Haiqi ^{[1
]}

Xu, Fan ^{[1
]}

Xia, Yonghong ^{[2
]}

机构：

[1] Chengdu Med Coll, Sch Publ Hlth, Dept Evidence Based Med & Social Med, Xindu Ave 783, Chengdu 610500, Sichuan, Peoples R China

[2] Qinghai Prov Traff Hosp, Dept Hepatobiliary Surg, Traff Lane 7, Xining 810001, Peoples R China

来源：

BMC GASTROENTEROLOGY | 2024年 / 24卷 / 01期

关键词：

Bile; Cholesterol gallstone; Pigment gallstone; High altitude; Metabolomics; Proteomics;

D O I：

10.1186/s12876-024-03422-5

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

BackgroundTo explore the pathogenesis of different subtypes of gallstones in high-altitude populations from a molecular perspective.MethodsWe collected bile samples from 20 cholesterol gallstone disease (CGD) patients and 20 pigment gallstone disease (PGD) patients. Proteomics analysis was performed by LC/MS DIA, while metabolomics analysis was performed by UPLC- Q-TOF/MS.ResultsWe identified 154 up-regulated and 196 down-regulated differentially expressed proteins, which were significantly enriched in neurodegenerative diseases, energy metabolism, amino acid metabolism etc. In metabolomics analysis, 20 up-regulated and 63 down-regulated differentially expressed metabolites were identified, and they were significantly enriched in vitamin B6 metabolism. Three pathways of integrated proteomics and metabolomics were significantly enriched: porphyrin and chlorophyll metabolism, riboflavin metabolism and aminoacyl-tRNA biosynthesis. Remarkably, 7 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.ConclusionThe findings of our metabolomics and proteomics analyses help to elucidate the underlying mechanisms of gallstone formation in high-altitude populations.

引用

页数：12