Daul-optical path observation system for tracking free-moving C. elegans

被引：0

作者：

Yu T. ^{[1
]}

Xu X. ^{[1
]}

Zhang S. ^{[2
]}

Zhang N. ^{[1
]}

Li H. ^{[3
]}

Wang X. ^{[3
]}

机构：

[1] School of Optoelectronic Engineering, Changchun University of Science and Technology, Changchun

[2] Changchun Yisheng Technology Co. ,Ltd, Changchun

[3] Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun

来源：

Guangxue Jingmi Gongcheng/Optics and Precision Engineering | 2024年 / 32卷 / 11期

关键词：

C; elegans; C. elegans observing system; daul-optical path imaging; microscopy imaging;

D O I：

10.37188/OPE.20243211.1676

中图分类号：

学科分类号：

摘要：

To overcome challenges related to a limited field of view and susceptibility to environmental fac⁃ tors impacting data accuracy, we have developed a free-moving Caenorhabditis elegans (C. elegans) dual-optical observation system. This system integrates both a microscopy imaging module and a large-field-of-view imaging module, allowing for the simultaneous capture of C. elegans microscope images and Petri dish images. By incorporating a switchable fluorescence module, the system meets the requirements for capturing C. elegans fluorescence images. Additionally, the system includes an XYZ three-axis platform to prevent vibration-triggered escape responses from C. elegans. We utilized this system to conduct imaging experiments on USAF1951 resolution plates and various C. elegans strains. The experimental results dem⁃ onstrated exceptional microscopy imaging resolution of less than 2.19 mm, vividly displaying C. elegans' organs, tissues, and cell boundaries in bright-field microscopy images, along with clear imaging of various fluorescent protein distributions. The large-field-of-view module, featuring uniform illumination and a mor⁃ phological algorithm, enables the collection of behavior and motion data from the C. elegans population. In summary, this system fulfills various C. elegans tracking requirements, exhibits significant stability and da⁃ ta accuracy, enhances the flexibility of C. elegans experiments, and lays a foundation for integrating C. ele⁃ gans tracking data across different scales. © 2024 Chinese Academy of Sciences. All rights reserved.

引用

页码：1676 / 1685

页数：9

共 16 条

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