Isolation and identification of efficient levoglucosan-assimilating microorganisms and effects of pretreatment on their utilization of pyrolysis liquids

被引:0
|
作者
Sun H. [1 ]
Li D.-X. [1 ]
Chen Y.-R. [1 ]
Zhao Y.-X. [1 ]
Liu J.-L. [2 ]
Wei M. [2 ]
机构
[1] Jiangsu Key Lab. for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing
[2] Institute of Chemical Industry of Forest Products, CAF, Nanjing
来源
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | 2016年 / 30卷 / 01期
关键词
Levoglucosan; Penicillium citrinum; Penicillium pinophilum; Pyrolysis liquid; Rhodopseudomonas sp;
D O I
10.3969/j.issn.1003-9015.2016.01.019
中图分类号
学科分类号
摘要
Four efficient levoglucosan-assimiliating microorganisms were screened using levoglucosan from cellulosis pyrolysis as carbon source, and they were identified as Penicillium pinophilum, Penicillium citrinum, Eurotium cristatum and Rhodopseudomonas sp based on morphology and ITS sequence analysis. The results show that the 2%-levoglucosan utilization efficiency of P. pinophilum, P. citrinum (4 d) and E. cristatum (7 d) are 100%, and that of Rhodopseudomonas sp is 52% (10 d). Rhodopseudomonas sp can be cultured in untreated pyrolysis liquids with utilization efficiency of 69% (14 d), while Penicillium pinophilum, Penicillium citrinum and Eurotium cristatum can be cultured in pretreated pyrolysis liquids (treated by 5% activated carbon+Ca(OH)2). The pretreated pyrolysis liquids were analyzed and the results show that pretreatment has obvious influence on the pyrolysis liquid. Hexamethylcyclotrisiloxane, 1, 2-Cyclopentanedione, 5-methylfurfural, 3-methyl-1, 2-Cyclopentanedione and hydroxymethylfuraneol are fully removed, and the loss rate of levoglucosan is 27.1%. © 2016, Zhejiang University. All right reserved.
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页码:127 / 132
页数:5
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