Electrokinetic Preconcentration and Label-Free Electrical Detection of SARS-CoV-2 RNA at a Packed Bed of Bioconjugated Microspheres

被引：0
|
作者：
Devasinghe, Sanduni U. [1 ]
Claus, Echo L. [1 ]
Strait, Madison E. [1 ]
Pagariya, Darshna [1 ]
Anand, Robbyn K. [1 ]
机构：
[1] Department of Chemistry, Iowa State University, Ames,IA,50011-1021, United States
来源：
ACS Sensors | 2024年 / 9卷 / 11期
关键词：
In this communication; we demonstrate the electrical detection of SARS-CoV-2 RNA at low femtomolar concentrations without labels or amplification reactions. Following its extraction from virus particles; the viral RNA was electrokinetically preconcentrated (100-fold) within a packed bed of probe-modified microbeads. This preconcentration was accomplished by counter-flow focusing of the RNA along an electric field gradient generated by faradaic ion concentration polarization (fICP). Hybridization of the 30 kb target RNA to the probe-modified beads sufficiently altered their surface charge to yield a measurable change in the ionic conductivity of the packed bed─a feature leveraged for electrical detection. When a single-stranded DNA probe was used; the sensitivity of this enrichment and sensing scheme was low picomolar. However; the utilization of an uncharged PNA probe improved the limit of detection to 3.4 × 106 viral copies/mL (22.5 fM SARS-CoV-2 RNA). These results are significant for three reasons. First; the sensitivity is remarkable; given the micrometer scale of both the beads and interstitial spaces. Additional gains in enrichment and sensitivity are anticipated as fundamental parametric studies and optimization are undertaken. Second; this study reveals the impact of the probe type on the sensitivity of microscale surface ion conduction (μSIC) sensors. Third; the RNA sensing approach has practical advantages including its utilization of off-the-shelf beads; a reagent-free approach; nonoptical readout; and low driving voltage; which render it amenable to point-of-care (POC) implementation. © 2024 American Chemical Society;
D O I：
10.1021/acssensors.4c00427
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页码：5776 / 5781
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