Determination of Ciprofloxacin in Urine Using Terbium Ion as Fluorescent Probe

Factors effecting the fluorescence characteristics of the reaction CPFX-Tb were studied thoroughly. The effect of the experimental conditions on the fluorescence intensity was defined. In the pH 6.0 media, CPFX-Tb complex fluorescence system can emit intrinsic fluorescence of Tb. The excitation and emission wavelengths are 328 nm and 545 nm. The linear range is 1.0 × 10-8 - 1.0 × 10-6 mol·L-1.The regression equationis F=1.57 + 4.87 × 108, c,r=0.999 9 with the detection limit of 5.0 × 10-9 mol·L-1. It has been satisfactory for the determination of trace ciprofloxacin in urine by using terbium ion as fluorescent probe.