COMPARISON OF VARIOUS METHODS TO DETERMINE KINETIC CONSTANTS FOR beta -GALACTOSIDASE IN SOLUBLE AND IMMOBILISED STATES.

The graphical method of H. Lineweaver-D. Burk, G. S. Eadie, C. S. Hanes and the 'direct linear plot' have been used to determine the kinetic constants of beta -galactosidase in soluble and immobilized states. Because some of these methods require a reciprocal concentration scale, the activity values obtained from dilute substrate solutions are weighted more than when using more concentrated substrates. However, in the case of immobilized enzymes the values from a dilute substrate are more afffected by diffusion from the bulk solution into the pores where the enzyme is fixed, and vice versa. Therefore it seems preferable to use methods with direct substrate and velocity scales. 11 refs.