Plantlet regeneration from decapitated embryonic axes of pigeonpea [Cajanus cajan (L.) Millsp.]

An efficient plant regeneration protocol for decapitated embryonic axes of pigeonpea was developed so that this explant can further be used for Agrobacterium mediated transformation. Multiple shoots were produced from 15 hr soaked seed derived embryo axes without apical dome on modified. Murashige and Skoog (MS) medium supplemented with BAP (1 mg L-1) and IAA (0.5 mg L-1). Regenerated shoots were rooted on MS medium supplemented with IBA (0.3 mg L-1). The plantlets, thus obtained, were hardened and successfully established in soil where they showed normal morphological characters and produced seeds.