Microfabricated glucose, lactate, glutamate and glutamine thin-film biosensors

被引：0
|
作者：
Schalkhammer, Th. [1 ]
Lobamaier, Ch. [1 ]
Ecker, B. [1 ]
Wakolbinger, W. [1 ]
Kynclova, E. [1 ]
Hawa, G. [1 ]
Pittner, F. [1 ]
机构：
[1] Institut fur biochemie und, molekulare Zellbiologie der Univ, Wien, Vienna, Austria
来源：
Sensors and Actuators, B: Chemical | 1994年 / B19卷 / 1 -3 pt 2期
关键词：
Glucose sensors - Ion exchangers - Microelectrodes - Polymerization - Thin films;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Glucose, glutamate and lactate oxidase as well as glutaminase were covalently immobilized on thin-film metal electrodes employing a polyimine gel obtained by polymerization of amines and aldehydes. The gel matrix having ion-exchange properties serves an excellent suppression layer for electrochemically interfering compounds. A glutamine sensor was obtained using the glutamate sensor in combination with highly purified immobilized glutaminase from Escherichia coli, pig kidney and Bacillus subtilis. Sensor thus obtained showed rapid response, high stability, excellent suppression of interfering substances and short regeneration time. These sensors were designed for fermentation control in bioreactors using flow-injection-analysis device (FIA). The sensors were also applied for assaying in plasma and whole blood.
引用
收藏
页码：587 / 591
相关论文
共 50 条
  • [1] MICROFABRICATED GLUCOSE, LACTATE, GLUTAMATE AND GLUTAMINE THIN-FILM BIOSENSORS
    SCHALKHAMMER, T
    LOBMAIER, C
    ECKER, B
    WAKOLBINGER, W
    KYNCLOVA, E
    HAWA, G
    PITTNER, F
    SENSORS AND ACTUATORS B-CHEMICAL, 1994, 19 (1-3) : 587 - 5591
  • [2] MINIATURIZED THIN-FILM GLUTAMATE AND GLUTAMINE BIOSENSORS
    MOSER, I
    JOBST, G
    ASCHAUER, E
    SVASEK, P
    VARAHRAM, M
    URBAN, G
    ZANIN, VA
    TJOUTRINA, GY
    ZHARIKOVA, AV
    BEREZOV, TT
    BIOSENSORS & BIOELECTRONICS, 1995, 10 (6-7): : 527 - 532
  • [3] Miniaturized thin film glutamate and glutamine biosensors
    Tyutrina, GV
    Zharikova, AV
    Zanine, VA
    Berezov, TT
    Mozer, I
    Jobst, G
    Aschauer, E
    Svasek, P
    Varahram, M
    Urban, G
    VOPROSY MEDITSINSKOI KHIMII, 1997, 43 (01): : 20 - 28
  • [4] Miniaturized thin film glutamate and glutamine biosensors
    Moser, I., 1600, Elsevier Science Ltd, Oxford, United Kingdom (10): : 6 - 7
  • [5] THIN-FILM CONDUCTOMETRIC BIOSENSORS FOR GLUCOSE AND UREA DETERMINATION
    SHULGA, AA
    SOLDATKIN, AP
    ELSKAYA, AV
    DZYADEVICH, SV
    PATSKOVSKY, SV
    STRIKHA, VI
    BIOSENSORS & BIOELECTRONICS, 1994, 9 (03): : 217 - 223
  • [6] Thin-film microbiosensors for glucose-lactate monitoring
    Jobst, G
    Moser, I
    Varahram, M
    Svasek, P
    Aschauer, E
    Trajanoski, Z
    Wach, P
    Kotanko, P
    Skrabal, F
    Urban, G
    ANALYTICAL CHEMISTRY, 1996, 68 (18) : 3173 - 3179
  • [7] LACTATE, GLUTAMATE AND GLUTAMINE BIOSENSORS BASED ON RHODINISED CARBON ELECTRODES
    WHITE, SF
    TURNER, APF
    BILITEWSKI, U
    SCHMID, RD
    BRADLEY, J
    ANALYTICA CHIMICA ACTA, 1994, 295 (03) : 243 - 251
  • [8] THIN-FILM CONDUCTOMETRIC ENZYME BIOSENSORS FOR THE DETERMINATION OF GLUCOSE AND UREA IN BLOOD
    SHULGA, AA
    DZYADEVICH, SV
    SOLDATKIN, AP
    PATSKOVSKII, SV
    STARODUB, NF
    STRIKHA, VI
    ELSKAYA, AV
    RUSSIAN ELECTROCHEMISTRY, 1993, 29 (08): : 1219 - 1224
  • [9] Thin-film amperometric multibiosensor for simultaneous determination of lactate and glucose in wine
    Shkotova, Lyudmyla V.
    Piechniakova, Nataliia Y.
    Kukla, Oleksandr L.
    Dzyadevych, Sergei V.
    FOOD CHEMISTRY, 2016, 197 : 972 - 978
  • [10] PERFORMANCE OF INTEGRATED GLUCOSE AND LACTATE THIN-FILM MICROBIOSENSORS FOR CLINICAL ANALYZERS
    URBAN, G
    JOBST, G
    ASCHAUER, E
    TILADO, O
    SVASEK, P
    VARAHRAM, M
    RITTER, C
    RIEGEBAUER, J
    SENSORS AND ACTUATORS B-CHEMICAL, 1994, 19 (1-3) : 592 - 596
12345