horC confers beer-spoilage ability on hop-sensitive Lactobacillus brevis ABBC45cc

被引：0

作者：

Iijima, K. ^{[1
,2
]}

Suzuki, K. ^{[1
]}

Ozaki, K. ^{[1
]}

Yamashita, H. ^{[1
]}

机构：

[1] Analytical Technology Laboratory, Asahi Breweries, Ltd., Ibaraki, Japan

[2] Analytical Technology Laboratory, Asahi Breweries, Ltd., 1-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan

来源：

Journal of Applied Microbiology | 2006年 / 100卷 / 06期

关键词：

Aims: To determine whether horC confers beer-spoilage ability and to evaluate the validity of horC as a trans-species genetic marker for differentiating the beer-spoilage ability of lactic acid bacteria (LAB). Methods and Results: Hop-sensitive Lactobacillus brevis ABBC45cc was transformed with an expression plasmid; pHYchorBC; containing putative multidrug resistance gene horC and its putative regulator horB; and the transformant was designated as ABBC45cc/pHYchorBC. As a control; ABBC45cc was transformed with pHYchorB that contains horB; and the transformed strain was designated as ABBC45cc/pHYchorB. As a result of beer-spoilage assay of these transformants; ABBC45cc/pHYchorBC exhibited beer-spoilage ability; whereas ABBC45cc/pHYchorB did not. Furthermore ABBC45 cc/pHYchorBC showed higher hop resistance than ABBC45 cc/pHYchorB; accounting for the differences in beer-spoilage ability observed between the two transformants. ABBC45cc/pHYchorBC also exhibited higher resistance to various structurally unrelated drugs; compared with ABBC45cc/pHYchorB. Conclusions: horC was shown to confer hop resistance and beer-spoilage ability on ABBC45cc by presumably encoding a multidrug transporter. Significance and Impact of the Study: The finding that horC plays an important role in hop resistance and beer-spoilage ability supports the validity of horC as a trans-species genetic marker for differentiating the beer-spoilage ability of LAB. © 2006 The Society for Applied Microbiology;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Journal article (JA)

引用

页码：1282 / 1288

共 7 条

[1] horC confers beer-spoilage ability on hop-sensitive Lactobacillus brevis ABBC45cc
Iijima, K
Suzuki, K
Ozaki, K
Yamashita, H
[J]. JOURNAL OF APPLIED MICROBIOLOGY, 2006, 100 (06) : 1282 - 1288
[2] Isolation of hop-sensitive variants from beer-spoilage Lactobacillus brevis strains
Suzuki, K
Koyanagi, M
Yamashita, H
[J]. JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS, 2004, 62 (02) : 71 - 74
[3] Genetic characterization of hop-sensitive variants obtained from beer-spoilage Lactobacillus brevis
Suzuki, K
Sami, M
Ozaki, K
Yamashita, H
[J]. JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS, 2005, 63 (01) : 5 - 10
[4] Genetic characterization of non-spoilage variant isolated from beer-spoilage Lactobacillus brevis ABBC45C
Suzuki, K
Koyanagi, M
Yamashita, H
[J]. JOURNAL OF APPLIED MICROBIOLOGY, 2004, 96 (05) : 946 - 953
[5] Isolation of a hop-sensitive variant of Lactobacillus lindneri and identification of genetic markers for beer spoilage ability of lactic acid bacteria
Suzuki, K
Iijima, K
Ozaki, K
Yamashita, H
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (09) : 5089 - 5097
[6] Comparative study of two plasmids, pRH45 and pRH 20690, isolated from beer-spoilage Lactobacillus brevis ABBC45 and L. lindneri DSM20690
Suzuki, K
Sami, M
Ozaki, K
Yamashita, H
[J]. JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS, 2005, 63 (01) : 11 - 16
[7] Classification and identification of strains of Lactobacillus brevis based on electrophoretic characterization of D-Lactate dehydrogenase:: Relationship between D-Lactate dehydrogenase and beer-spoilage ability
Takahashi, T
Nakakita, Y
Sugiyama, H
Shigyo, T
Shinotsuka, K
[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (05) : 500 - 506

← 1 →