Grafting Glycopolymer Brushes onto Gigaporous Polystyrene Microspheres via Atom Transfer Radical Polymerization

被引：0

作者：

Qu J. ^{[1
]}

Peng W. ^{[1
]}

Lin Y. ^{[1
]}

Li D. ^{[1
]}

Li J. ^{[1
]}

Liu J. ^{[1
]}

Zhang X. ^{[1
]}

机构：

[1] College of Chemical Engineering, China University of Petroleum (East China), Qingdao

来源：

Gaofenzi Cailiao Kexue Yu Gongcheng/Polymeric Materials Science and Engineering | 2020年 / 36卷 / 05期

关键词：

Atom transfer radical polymerization; Diacetone-D-glucose; Gigaporous polystyrene microspheres; Glypolymer brushes;

D O I：

10.16865/j.cnki.1000-7555.2020.0021

中图分类号：

学科分类号：

摘要：

Gigaporous polystyrene (PS) microspheres were characterized with high mechanical strength, good chemical stability and excellent mass transfer property. However, the hydrophobic surfaces of PS microspheres limit their application in bioseparation medium except by further hydrophilic modification. In this study, glycopolymer brushes grafting gigaporous PS microspheres (PS-PMAGlu) were successfully fabricated through the three-step reaction. Bromoisobutyryl groups were first coupled on PS microspheres through Friedel-crafts reaction to prepare a solid atom transfer radical polymerization (ATRP) initiator (PS-Br). Then, poly (3-O-methallyl diacetone-D-glucose) brushes were grafted onto PS-Br microspheres (PS-PMADAGlu) via ATRP. Finally, PS-PMAGlu microspheres were obtained by formic acid hydrolysis to remove the protective groups of glucose moiety. In order to realize the control of grafting amounts of glycopolymer brushes, the grafting conditions were optimized by investigating the solvents, ligands, catalysts and temperature, and the maximum grafting amount of glycopolymer brushes is 490.8 mg/g dry microspheres. PS-PMAGlu microspheres were characterized by Fourier transform infrared (FT-IR) spectra, X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM), mercury porosimetry measurements, water contact angle measurements, and protein adsorption experiments. After grafting glycopolymer brushes, the hydrophilicity of PS microspheres is greatly improved and their gigaporous structures are well maintained. The water contact angle of PS-PMAGlu microspheres decreases significantly from 124°to 42°. The adsorption amount of bovine serum albumin (BSA) on PS-PMAGlu microspheres is 10.2 times lower than that on PS microspheres. In addition, the flexible glycopolymer brushes can serve as spacer arm in the following derivatization, which will facilitate the interaction between ligands and biomacromolecules. All the results indicate that glycopolymer brushes grafting PS microspheres are promising in high-speed protein chromatography after further derivatization. © 2020, Editorial Board of Polymer Materials Science & Engineering. All right reserved.

引用

页码：12 / 19

页数：7

共 15 条

[1] Hoffman A S, Stayton P S, Bennett L E, Et al., Conjugates of stimuli-responsive polymers and proteins, Progress in Polymer Science, 32, pp. 922-932, (2007)
[2] Yao M, Ning T M, Jiang Z G., Preparation of heat-resisting polystyrene microspheres, Polymer Materials Science & Engineering, 33, 10, pp. 121-124, (2017)
[3] Afeyan N B, Gordon N F, Mazsaroff I, Et al., Flow-through particles for the high-performance liquid chromatographic separation of biomolecules: Perfusion chromatography, Journal of Chromatography A, 519, pp. 1-29, (1990)
[4] Li N H, Benson J R, Kitagawa N., Polymeric microbeads
[5] Zhou W Q, Gu T Y, Su Z G, Et al., Synthesis of macroporous poly (styrene-divinyl benzene) microspheres by surfactant reverse micelles swelling method, Polymer, 48, pp. 1981-1988, (2007)
[6] Qu J B, Zhou W Q, Wei W, Et al., An effective way to hydrophilize gigaporous polystyrene microspheres as rapid chromatographic separation media for proteins, Langmuir, 24, pp. 13646-13652, (2008)
[7] Qu J B, Zhou W Q, Wei W, Et al., Chemical modification and characterization of gigaporous polystyrene microspheres as rapid separation of proteins base supports, Journal of Polymer Science Part A: Polymer Chemistry, 46, pp. 5794-5804, (2008)
[8] Qu J B, Shou H H, Li J, Et al., Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support, Reactive & Functional Polymers, 72, pp. 606-612, (2012)
[9] Qu J B, Xu Y L, Liu J Y, Et al., Chemical coating polysaccharide on gigaporous polystyrene microspheres as a high-speed protein chromatography matrix, Polymer Engineering and Science, 56, pp. 1407-1414, (2016)
[10] Qu J B, Huan G S, Chen Y L, Et al., Coating gigaporous polystyrene microspheres with cross-linked poly(vinyl alcohol) hydrogel as a rapid protein chromatography matrix, ACS Applied Materials & Interfaces, 6, pp. 12752-12760, (2014)

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