Headspace solid-phase microextraction-gas chromatography-mass spectrometry for analysis of key volatile compounds in aromatic water from ponkan

For the fist time, key volatile compounds in the headspace of aromatic water from ponkan has been analyzed using headspace solid -phase microextraction (HS -SPME) combined with gas chromatography -mass spectrometry (GC-MS). The effects of SPME fiber (PDMS/DVB 65 μm, CAR/PDMS 75 μm and DVB/CAR/PDMS 50/30 μm), adsorption temperature (30~50℃), adsorption time (5~30min) and sample volume (2~10 mL) were studied to develop HS-SPME condition for obtaining the highest extraction efficiency. For the headspace volatile extraction, the optimum conditions were: DVB/CAR/PDMS fiber, 8 ml of sample volume, adsorption at 40octanol, 6.2℃ for 20 min. The main volatile flavor compounds were: d-limonene, 40.85%; ethanol, 17.17%; a-terpineol, 15.61%; 1-0%; β-myrcene, 3.48%; 3-methyl-1-butanol, 3.01%; β-linalool, 2.93%; naphthalene, 2.88%; ethyl caprylate, 2.34%; α-pinene, 1.47%. Ten kinds of compounds accounted for 95.94% of total peak area. Relative standard deviation (RSD) for three replicate analyses was found to be less than 7.0% and confirmed the feasibility of the HS -SPME -GC/MS technique for headspace analysis of key volatile compounds in aromatic water from ponkan. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.