Isolation of Endophytic Bacteria from Astragalus membranaceus (Fisch.) Bge. and Analysis of Their Secondary Metabolites

被引：0
|
作者：
Zhai L. [1 ,2 ]
Xu H. [1 ,2 ]
Li W. [1 ,2 ]
Zheng X. [1 ,2 ]
Zheng C. [1 ,2 ]
机构：
[1] Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin
[2] College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin
来源：
Journal of Chinese Institute of Food Science and Technology | 2019年 / 19卷 / 03期
关键词：
Antimicrobial activity; Astragalus membranaceus(Fisch.) Bge; Endophytic bacteria; Secondary metabolites;
D O I：
10.16429/j.1009-7848.2019.03.037
中图分类号：
学科分类号：
摘要：
Endophytic bacteria from Astragalus membranaceus (Fisch.) Bge. and their secondary metabolites were investigated in order to find novel resources. Endophytic bacteria were isolated from Astragalus membranaceus (Fisch.) Bge. by using the methods of plate cultivation, and the antimicrobial activities of endophytic bacteria's secondary metabolites were carried out by paper disc diffusion method. Meanwhile, with formononetin and Astragalus membranaceus (Fisch.) Bge. real samples as reference substances, the secondary metabolites of these endophytic bacteria were analysis by HPLC, and the target endophytic bacteria were identified by the 16S rDNA sequence homology and their physiological and biochemical characteristics. The results indicated that 31 strains of endophytic bacteria (86.1% of the total strains isolated) showed the different antimicrobial activities, and the secondary metabolites of 2 stains had the similar or new active metabolites as their hosts, then the target bacterium HX8 was identified as Bacillus methylotrophicus. As a conclusion, Diverse antimicrobial natural products are existed in the secondary metabolites of endophytic bacteria from Astragalus membranaceus (Fisch.) Bge., and HX8 can product the similar or new active metabolites as its host which provided a new thought and way for using endophytic bacteria to ferment and produce the components of Astragalus membranaceus (Fisch.) Bge. © 2019, Editorial Office of Journal of CIFST. All right reserved.
引用
收藏
页码：299 / 305
页数：6
相关论文
共 19 条
  • [1] Natasa R., Borut S., Endophytic fungi-The treasure chest of antibacterial substances, Phytomedicine, 19, 14, pp. 1270-1284, (2012)
  • [2] Bhore S.J., Komathi V., Types of endophytic bacteria associated with traditional medicinal plant Lantana camara Linn, Phcog J, 4, 32, pp. 20-23, (2012)
  • [3] Arnold A.E., Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers, Fungal Biology Reviews, 21, 2-3, pp. 51-66, (2007)
  • [4] Matsumura E., Fukuda K., A comparison of fungal endophytic community diversity in tree leaves of rural and urban temperate forests of Kanto district, eastern Japan, Fungal Biology, 117, 3, pp. 191-201, (2013)
  • [5] Yang G.D., Gao R., Wang Y., Et al., Determination of swainsonine in the endophytic Undifilum fungi by high-performance liquid chromatography with evaporative light-scattering detector, Toxicon, 60, 1, pp. 44-49, (2012)
  • [6] Gunter B., Ste'Phane C., Birgit M., Et al., Metabolic potential of endophytic bacteria, Current Opinion in Biotechnology, 27, pp. 30-37, (2014)
  • [7] Schulz B., Boyle C., Draeger S.-F., Et al., Endophytic fungi: a source of novel biologically active secondary metabolites, Mycological Research, 106, 9, pp. 996-1004, (2002)
  • [8] Schulz B., Boyle C., The endophytic continuum, Mycological Research, 109, 6, pp. 661-686, (2005)
  • [9] Chen J., Li Y., Yang L.Q., Et al., Biological activities of flavonoids from pathogenic-infected Astragalus adsurgens, Food Chemistry, 131, 2, pp. 546-551, (2012)
  • [10] Wang X.F., Li Y.L., Yang X.J., Et al., Astragalus polysaccharide reduces inflammatory response by decreasing permeability of LPS-infected Caco2 cells, International Journal of Biological Macromolecules, 60, pp. 347-352, (2013)
12