Multiplexed sandwich immunoassays using electrochemiluminescence imaging resolved at the single bead level

被引：0
|
作者：
Deiss, Frédérique [1 ]
LaFratta, Christopher N. [2 ]
Symer, Matthew [2 ]
Blicharz, Timothy M. [2 ]
Sojic, Neso [1 ]
Walt, David R. [2 ]
机构：
[1] Université Bordeaux, Institut des Sciences Moléculaires, ENSCPB, 16 Avenue Pey-Berland, 33607 Pessac, France
[2] Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, MA 02155, United States
来源：
Journal of the American Chemical Society | 2009年 / 131卷 / 17期
关键词：
A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is presented. This platform demonstrates the possibility of performing highly multiplexed assays using ECL because all the individual sensing beads in the array are simultaneously imaged and individually resolved by ECL. Duplex and triplex assay results are demonstrated as well as a cross reactivity study. © 2009 American Chemical Society;
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摘要：
Journal article (JA)
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页码：6088 / 6089
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