Study on cell cycle using fluorescence lifetime imaging microscopic system based on a streak camera

被引：0

作者：

Wang Y. ^{[1
]}

Zhao L. ^{[2
]}

Chen T. ^{[3
]}

Xu G. ^{[1
]}

Pliss A. ^{[2
]}

Ohulchanskyy T.Y. ^{[2
]}

Prasad P.N. ^{[2
]}

Qu J. ^{[1
]}

Niu H. ^{[1
]}

机构：

[1] Key Lab. of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Institute of Optoelectronics, Shenzhen University, Shenzhen

[2] Institute for Lasers, Photonics, and Biophotonics, State University of New York at Buffalo

[3] Key Laboratory of Laser Life Science of Ministry of Education and Institute of Laser Life Science, South China Normal University, Guangzhou

来源：

Zhongguo Jiguang/Chinese Journal of Lasers | 2011年 / 38卷 / 03期

关键词：

Cell cycle; Fluorescence lifetime imaging; HeLa cell; Imaging systems; Streak camera;

D O I：

10.3788/CJL201138.0308024

中图分类号：

学科分类号：

摘要：

The fluorescence lifetime of the HeLa cells which are transfected with green fluorescent proteins during the cell cycle is investigated using fluorescence lifetime imaging microscopic system based on a streak camera. Experimental results show that fluorescence lifetime of HeLa cells is between 2.50 ns and 3.00 ns during different processes of cell cycle. The fluorescence lifetime of the mitosis cell drops to 2.82 ns from 2.86 ns within one hour, and it further drops to 2.78 ns from 2.82 ns during the roughly eight hours of the pre-DNA-synthetic phase (G1-phase). The difference of the fluorescence lifetime implies that the macromolecular concentration in the nucleoplasm of the cell nucleus changes throughout the cell cycle, the study of which is significant to the understanding of macromolecular processes, kinetics and concentrations in the nucleoplasm of the cell nucleus throughout the cell cycle, as well as the regulation of cell cycles.

引用

共 19 条

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