Detection of Salmonella in Food by Visualized Saltatory Rolling Circle Amplification Technique

被引：0
|
作者：
Zhang Y. [1 ]
Yuan N. [2 ]
Li J. [2 ]
Zhang M. [1 ]
Ma X. [1 ]
Zhang W. [1 ,2 ,3 ]
机构：
[1] College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, Hebei
[2] College of Science and Technology, Hebei Agricultural University, Cangzhou, 061100, Hebei
[3] College of Life Sciences, Hebei Agricultural University, Baoding, 071001, Hebei
来源：
Journal of Chinese Institute of Food Science and Technology | 2020年 / 20卷 / 09期
关键词：
Detection; Food; Salmonella; Saltatory rolling circle amplification (SRCA); Stn;
D O I：
10.16429/j.1009-7848.2020.09.035
中图分类号：
学科分类号：
摘要：
A new type of vitro nucleic acid isothermal amplification method-saltatory rolling circle amplification (SRCA) was established to detect Salmonella. The primers were designed using the specific gene stn of Salmonella and the specificity was verified. The performance of the assay was evaluated with respect to sensitivity and the detection limit of artificially contaminated chicken was tested. In addition, the sensitivity, specificity and accuracy were evaluated by testing a variety of real food samples. Results: All Salmonella presented positive results, but non-Salmonella gave negative results, indicating good specificity of the primer. The sensitivity of SRCA method was 5.6×100 fg/μL for DNA, and the detection limit was 3.8×101 CFU/mL. The sensitivity of PCR method was 5.6×102 fg/μL, and the detection limit was 3.8×103 CFU/mL. In addition, the detection rate of this method for detecting 30 real samples was 13.33%. Compared with the GB 4789.4-2016, the sensitivity, specificity and accuracy of SRCA were 100.00%, 96.30% and 96.67%, respectively. Conclusions: The method has the advantages of simple operation, high specificity, high sensitivity, low detection limit, low cost and low equipment requirement, and is suitable for the rapid detection of Salmonella in food. © 2020, Editorial Office of Journal of CIFST. All right reserved.
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页码：304 / 311
页数：7
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