Analysis of absorbed components in rat plasma after oral administration of quasi estrogen fraction of cistanche by HPLC-ESI-Q-TOF MS/MS

To analyze and identify the constituents in rat plasma after oral administration of the active fraction of cistanche, the components in rat plasma were detected by high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (HPLC-Q-TOF MS). Structures of the components were elucidated by comparing their retention times and MS/MS spectra with those of reference compounds and reported data in the literatures. 13 chemical compositions are identified, including 6prototype components, such as Geniposidic acid, Salidroside, Cistanoside A, Echinacoside, Acteoside, Cistanoside D, 7 metabolites components, such as 3, 4-Dihydroxyphenylethanol glycosides, methylation acteoside, acetylation Cistanoside G, 3, 4-Dihydroxyphenylethanol, acetyl tubuloside B, Cistanoside B glucuronide metabolite and methylation-3, 4Dihydroxyphenylethanol. The results can provide essential data for illuminate the therapeutic basis of quasi estrogen regulation activity of cistanche. ©, 2015, Chinese Society for Mass Spectrometry. All right reserved.