Preparation of biodegradable and thermoresponsive enzyme-polymer conjugates with controllable bioactivity via RAFT polymerization

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[1] Liu, Huihui
[2] Zhang, Jizhen
[3] Luo, Xiong
[4] Kong, Na
[5] Cui, Liang
[6] Liu, Jingquan
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Liu, J. (jliu@qdu.edu.cn) | 1600年 / Elsevier Ltd卷 / 49期
关键词：
The preparation of biodegradable and thermoresponsive enzyme-polymer bioconjugates with controllable enzymatic activity via reversible addition-fragmentation chain transfer (RAFT) polymerization and amidation conjugation reaction is presented. A new 2-mercaptothiazoline ester functionalized RAFT agent with intra-disulfide linkage was synthesized and used as chain transfer agent (CTA) to generate a biocompatible homopolymer; poly(ethyleneglycol) acrylate (polyPEG-A) and a thermoresponsive copolymer of poly(ethyleneglycol) acrylate with di(ethyleneglycol)ethyl ether acrylate [poly(PEG-A-co-DEG-A)]. These biodegradable and thermoresponsive polymers were then conjugated to the surface of glucose oxidase (GOx) under mild condition to afford the biodegradable and thermoresponsive enzyme-polymer conjugates. Cleavage of the polymer chains from the GOx surface obviously recovered the enzymatic activity. The thermoresponsive test of GOx-poly(PEG-A-co-DEG-A) revealed that the bioconjugate exhibited regular enzymatic activity fluctuation upon the temperature change below or above the lower critical solution temperature (LCST). The as-prepared enzyme-polymer conjugates were also characterized using 1H NMR; UV-vis spectroscopy; polyacrylamide gel electrophoresis (PAGE) and biocatalytic activity tests. These smart enzyme-polymer conjugates would envision promising applications in biotechnology and biomedicine. © 2013 Elsevier Ltd. All rights reserved;
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