Biochemical characterization of an organic solvent- and salt-tolerant xylanase and its application of arabinoxylan-oligosaccharides production from corn fiber gum

被引：0
|
作者：
Academy of National Food and Strategic Reserves Administration, No 11 Baiwanzhuang Avenue, Xicheng District, Beijing [1 ]
100037, China
机构：
来源：
Int. J. Biol. Macromol. | 2024年
关键词：
A endo-xylanase; of the glycoside hydrolase family 10 from Schizophyllum commune DB01; was expressed in P. pastoris. Recombinant xylanase (Scxyn5) retained above 80 % maximum activity in 10 % dimethyl sulfoxide and retained 90 % maximum activity in 5 M NaCl on the substrate of birchwood xylan. The effect of NaCl on the catalytic activity of Scxyn5 was significantly different toward various substrates; which was caused by the difference of monosaccharide composition and sturcture of the substrates. Furthermore; when corn fiber gum (CFG) was used as a substrate; the catalytic activity of Scxyn5 increased by 1.3–2.03 times in 1–5 M NaCl. Based on response surface methodology; the highest catalytic activity of Scxyn5 in hydrolyzing CFG were achieved with enzymatic temperature of 50 °C; pH value of 6.0; and 4 M NaCl. These properties of Scxyn5 suit the arabinoxylan-oligosaccharides (AXOs) preparation from CFG and some other potential applications in food industry. © 2024 Elsevier B.V;
D O I：
10.1016/j.ijbiomac.2024.136146
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 34 条
  • [1] Biochemical characterization of a novel xylanase from Paenibacillus barengoltzii and its application in xylooligosaccharides production from corncobs
    Liu, Xueqiang
    Liu, Yu
    Jiang, Zhengqiang
    Liu, Haijie
    Yang, Shaoqing
    Yan, Qiaojuan
    [J]. FOOD CHEMISTRY, 2018, 264 : 310 - 318
  • [2] Characterization of an organic solvent-tolerant lipase from Haloarcula sp G41 and its application for biodiesel production
    Li, Xin
    Yu, Hui-Ying
    [J]. FOLIA MICROBIOLOGICA, 2014, 59 (06) : 455 - 463
  • [3] An organic solvent tolerant lipase from Serratia marcescens ECU1010: Biochemical characterization and practical application
    Xu, Jian-He
    Zhao, Li-Li
    Zhao, Jian
    Pan, Jiang
    Wang, Zhi-Long
    [J]. JOURNAL OF BIOTECHNOLOGY, 2008, 136 : S51 - S51
  • [4] Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophilia NYW-81 and its application in Japanese soy sauce fermentation
    Wakayama, M
    Yamagata, T
    Kamemura, A
    Bootim, N
    Yano, S
    Tachiki, T
    Yoshimune, K
    Moriguchi, M
    [J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2005, 32 (09) : 383 - 390
  • [5] Characterization of an organic solvent-tolerant lipase from Haloarcula sp. G41 and its application for biodiesel production
    Xin Li
    Hui-Ying Yu
    [J]. Folia Microbiologica, 2014, 59 : 455 - 463
  • [6] Purification and characterization of an organic solvent-tolerant lipase from Pseudomonas aeruginosa LX1 and its application for biodiesel production
    Ji, Qingchun
    Xiao, Sujing
    He, Bingfang
    Liu, Xiaoning
    [J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2010, 66 (3-4) : 264 - 269
  • [7] Novel Salt-Tolerant Xylanase from a Mangrove-Isolated Fungus Phoma sp. MF13 and Its Application in Chinese Steamed Bread
    Wu, JingJing
    Qiu, Conghua
    Ren, Yaxin
    Yan, Renxiang
    Ye, Xiuyun
    Wang, Guozeng
    [J]. ACS OMEGA, 2018, 3 (04): : 3708 - 3716
  • [8] Biochemical characterization of an organic solvent-tolerant glycosyltransferase from Bacillus licheniformis PI15 with potential application for raspberry ketone glycoside production
    Li, Bingfeng
    Fan, Bo
    Fan, Jingping
    Chang, Siyuan
    Pan, Xin
    Wang, Yinan
    Wu, Yaming
    Song, Junsong
    He, Xuejun
    [J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2020, 67 (02) : 249 - 256
  • [9] Production and Characterization of a Halo-, Solvent-, Thermo-tolerant Alkaline Lipase by Staphylococcus arlettae JPBW-1, Isolated from Rock Salt Mine
    Chauhan, Mamta
    Garlapati, Vijay Kumar
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2013, 171 (06) : 1429 - 1443
  • [10] Production and Characterization of a Halo-, Solvent-, Thermo-tolerant Alkaline Lipase by Staphylococcus arlettae JPBW-1, Isolated from Rock Salt Mine
    Mamta Chauhan
    Vijay Kumar Garlapati
    [J]. Applied Biochemistry and Biotechnology, 2013, 171 : 1429 - 1443
1234