Separation, identification and toxigenicity of algae in bloom of Meiliang Bay of Lake Taihu

被引：0
|
作者：
Li X. [1 ]
Yang F. [1 ]
Yin L. [1 ]
Liang G. [1 ]
Li Y. [1 ]
Pu Y. [1 ]
机构：
[1] Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Southeast University
来源：
Dongnan Daxue Xuebao (Ziran Kexue Ban)/Journal of Southeast University (Natural Science Edition) | 2010年 / 40卷 / 06期
关键词：
Algae; Eutrophication; High performance liquid chromatography (HPLC); Lake Taihu; Microcystins; Whole-cell polymerase chain reaction (PCR);
D O I：
10.3969/j.issn.1001-0505.2010.06.039
中图分类号：
学科分类号：
摘要：
Algae were isolated from Meiliang Bay of Lake Taihu by dilution culture method and the antibiotics were applied to inhibit the growth of bacteria. The genus and toxigenicity of algae were analyzed by whole-cell polymerase chain reaction (PCR), high performance liquid chromatography (HPLC) and real-time quantitative PCR (RTQ-PCR) method. The results show that two algae strains are successfully isolated from Meiliang Bay of Lake Taihu bloom named as TH1 and TH2. The Phycocyanin intergenic spacer (PC-IGS) gene and 16S ribosoma deoxyribonucleic acid of Microcystis(Micr 16S rDNA) gene are amplified as positive in both algae strains and the Microcystin Synthetase B gene (mcyB) is only found in TH1 strain in whole-cell PCR. Microcystins-LR produced by TH1 strain is detected as 0.594 μg in total and 0.085 μg in extracellular per 108 cells in 15 d culture, which is 61.39% and 86.09% of MC-LR produced by Microcystis aeruginosa strain, respectively. A negative result of MC-LR is obtained in TH2 strain. The express intensity of mcyB in mRNA level of TH1 is 5.9% of Microcystis aeruginosa. It is concluded that two isolated algae strains from the Meiliang Bay of Lake Taihu are Microcystis and one of them has stronger ability of MC-LR production. Water pollution of toxic Microcystis exists in the area.
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页码：1332 / 1336
页数：4
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