Resolution enhancement in solid-state NMR of oriented membrane proteins by anisotropic differential linebroadening

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作者：
Vosegaard, Thomas [1 ]
Bertelsen, Kresten [1 ]
Pedersen, Jan M. [1 ]
Thøgersen, Lea [1 ]
Schiøtt, Birgit [1 ]
Tajkhorshid, Emad [2 ]
Skrydstrup, Troels [1 ]
Nielsen, Niels Chr. [1 ]
机构：
[1] Center for Insoluble Protein Structures (inSPIN), Department of Chemistry, University of Aarhus, Langelandsgade 140, DK-8000 Aarhus C, Denmark
[2] Department of Biochemistry, Beckman Institute, University of Illinois at Urbana-Champaign, 405 North Mathews, Urbana, IL 61801, United States
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| 1600年 / American Chemical Society, Columbus, OH 43210-3337, United States卷 / 130期
关键词：
We demonstrate that a significant improvement in the spectral resolution may be achieved in solid-state NMR experiments of proteins in inhomogeneously disordered oriented lipid bilayers. Using 1H homonuclear decoupling instead of standard 1H heteronuclear decoupling; the 15N line widths may be reduced by up to seven times for such samples. For large oriented membrane proteins; such resolution enhancements may be crucial for assignment and structural interpretation. Copyright © 2008 American Chemical Society;
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