Engineered Saccharomyces cerevisiae that produces 1,3-propanediol from D-glucose

被引：0
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作者：
Rao, Z. [1 ,2 ]
Ma, Z. [1 ]
Shen, W. [1 ]
Fang, H. [1 ]
Zhuge, J. [1 ]
Wang, X. [1 ,2 ]
机构：
[1] Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, China
[2] School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province 214122, China
来源：
Journal of Applied Microbiology | 2008年 / 105卷 / 06期
关键词：
Aims: Saccharomyces cerevisiae is a safe micro-organism used in fermentation industry. 1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensively chemical synthesis. The aim of this study is to engineer a S. cerevisiae strain that can produce 1,3-propanediol at low cost. Methods and Results: By using d-glucose as a feedstock, S. cerevisiae could produce glycerol, but not 1,3-propanediol. In this study, we have cloned two genes yqhD and dhaB required for the production of 1,3-propanediol from glycerol, and integrated them into the chromosome of S. cerevisiae W303-1A by Agrobacterium tumefaciens-mediated transformation. Both genes yqhD and dhaB functioned in the engineered S. cerevisiae and led to the production of 1,3-propanediol from d-glucose. Conclusion: Saccharomyces cerevisiae can be engineered to produce 1,3-propanediol from low-cost feedstock d-glucose. Significance and Impact of the Study: To our knowledge, this is the first report on developing S. cerevisiae to produce 1,3-propanediol by using A. tumefaciens-mediated transformation. This study might lead to a safe and cost-efficient method for industrial production of 1,3-propanediol. © 2008 The Authors;
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页码：1768 / 1776
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