Protein-imprinted soft-wet gel composite microspheres with magnetic susceptibility. II. Characteristics

被引：0
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作者：
Lu, Shulai [1 ,2 ]
Cheng, Guoxiang [1 ]
Pang, Xingshou [1 ]
机构：
[1] School of Materials Science and Engineering, Tianjin University, Tianjin, China
[2] Research Institute of Juin, Petrochemical Company, PetroChina Company Limited, Jilin, China
来源：
Journal of Applied Polymer Science | 1600年 / 99卷 / 05期
关键词：
Protein-imprinted soft-gel composite microspheres with magnetic susceptibility (MS-PIGMs) were prepared by inverse suspension polymerization using Fe3O4 particles as magnetically susceptible component and bovine serum albumin and lysozyme (Lyz) as templates; respectively. The average content of magnetically susceptible component (Fe 3O4) inside MS-PIGMs was determined using thermogravimetric analyzer; and the magnetic characteristics of MS-PIGMs were measured by vibrating sample magnetometer. The results showed that the resulting MS-PIGMs had a certain magnetic response to external magnetic fields; and their average content of Fe3O4 was 2.08%. Their recognition specificity was investigated using BSA and Lyz as both templates and control molecules and characterized by high-performance liquid chromatography; and the mechanism of imprinting and recognition was analyzed. It was shown that the resulting BSA imprinted soft-gel composite microspheres with magnetic susceptibility (BSA-PIGMs) and Lyz imprinted soft-gel composite microspheres with magnetic susceptibility (Lyz-PIGMs). All exhibited good recognition selectivity for their templates; and the relative separation factor (β) was 4.75 and 5.88; respectively. The recognition selectivity of MS-PIGMs to their templates depended mainly on the synergic action of a large quantity of hydrogen binding being caused by complementation and very close contact of outer surface of proteins with inner surface of imprinting cavities. © 2005 Wiley Periodicals; Inc;
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页码：2401 / 2407
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