Utilizing alternative in vivo animal models for food safety and toxicity: A focus on thermal process contaminant acrylamide

Rodent models have traditionally been used to assess the toxicity of food chemicals, but this approach is costly, time-consuming, and raises ethical concerns. Alternatively, non-mammalian models such as Drosophila melanogaster, Danio rerio, and Caenorhabditis elegans have been shown to be suitable for studying the toxicity of food hazards. Their advantages include low cost, short life cycles, adaptability to high-throughput screening, and adherence to the 3R principles of replacement, reduction, and refinement. These models have been extensively studied in the context of acrylamide toxicity, a common food contaminant. This article comprehensively reviews the biological characteristics of non-mammalian models, recent advances and challenges in acrylamide toxicity research using these models, and explores the potential of natural plant compounds in ameliorating acrylamide toxicity. The review aims to guide research using non-mammalian models for food safety assessment.