Detection of genetic modification in cured tobacco leaf: Proficiency testing using polymerase chain reaction-based methods

被引：0
|
作者：
Gadani, Ferruccio [1 ]
Ward, Martin [2 ]
Black, Sue [2 ]
Harris, Neil [3 ]
McDowell, David [3 ]
Bolev, Nick [3 ]
机构：
[1] Philip Morris International, Research and Development, Quai Jeanrenaud 56, CH 2000 Neuchâtel, Switzerland
[2] Advanced Technologies (Cambridge) Ltd., 210 Cambridge Science Park, Cambridge CB4 0WA, United Kingdom
[3] LGC Ltd., Queens Rd, Teddington, Middlesex TW11 0LY, United Kingdom
来源：
Journal of AOAC International | 2006年 / 89卷 / 03期
关键词：
The Cooperation Centre for Scientific Research Relative to Tobacco (CORESTA; Paris; France) Task Force Genetically Modified Tobacco - Detection Methods investigated the performance of qualitative and quantitative methods based on the polymerase chain reaction (PCR) for the detection and quantitation of genetically modified (GM) tobacco. In the 4 successful rounds of proficiency testing; the cauliflower mosaic virus 35S RNA promoter (CaMV 35S) and the Agrobacterium tumefaciens nopaline synthase terminator (NOS) were selected as target sequences. Blind-coded reference materials containing from 0.1 to 5.0% and from 0.15 to 4% GM tobacco were used in 2 rounds of qualitative and quantitative PCR; respectively. Eighteen laboratories from 10 countries participated in this study. Considering all methods and 2 rounds; the different laboratories were able to detect GM tobacco at the 0.1% level in 46 out of 58 tests in qualitative assays. The results of the proficiency test indicate that both end point screening and real-time quantitative methods are suitable for the detection of genetically modified organisms in tobacco leaf samples having a GM content of 0.1% or higher. The CORESTA proficiency study represents a first step towards the interlaboratory evaluation of accuracy and precision of PCR-based GM tobacco detection; which may lead to the harmonization of analytical procedures and to the enhancement of comparability of testing results produced by different laboratories;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：693 / 707
相关论文
共 50 条
  • [1] Detection of genetic modification in cured tobacco leaf: Proficiency testing using polymerase chain reaction-based methods
    Gadani, F
    Ward, M
    Black, S
    Harris, N
    McDowell, D
    Boley, N
    JOURNAL OF AOAC INTERNATIONAL, 2006, 89 (03) : 693 - 707
  • [2] Polymerase chain reaction-based detection of lymphatic filariasis
    Fischer, P
    Boakye, D
    Hamburger, J
    MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2003, 192 (01) : 3 - 7
  • [3] Polymerase chain reaction-based detection of bacteria in semen
    Jarvi, K
    Dumitru, I
    Lacroix, JM
    Heritz, D
    Jain, A
    Mittelman, MW
    FERTILITY AND STERILITY, 1996, 66 (03) : 463 - 467
  • [4] Polymerase chain reaction-based detection of lymphatic filariasis
    Peter Fischer
    Daniel Boakye
    Joseph Hamburger
    Medical Microbiology and Immunology, 2003, 192 : 3 - 7
  • [5] Comparison of three polymerase chain reaction-based methods for the detection of Listeria monocytogenes in food
    Kaclíková, E
    Oravcová, K
    Kuchta, T
    Pangallo, D
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2004, 12 (02): : 107 - 113
  • [6] Polymerase chain reaction-based methods for the detection of heat-resistant ascomycetous fungi
    Yaguchi, Takashi
    MYCOSCIENCE, 2023, 64 (02) : 47 - 54
  • [7] Polymerase chain reaction-based methods of DNA methylation analysis
    Liu, ZJ
    Maekawa, M
    ANALYTICAL BIOCHEMISTRY, 2003, 317 (02) : 259 - 265
  • [8] Role of polymerase chain reaction-based viral detection in pterygia
    Krishnan, Janani Madhuravasal
    Rajagopal, Rama
    Lakshmipathy, Dhanurekha
    Agarwal, Shweta
    Anand, A.
    Therese, Lily
    Thangam, Aishwarya
    Madhavan, Hajib Narahari Rao
    INDIAN JOURNAL OF OPHTHALMOLOGY, 2023, 71 (02) : 458 - 463
  • [9] Polymerase chain reaction-based detection of Xanthomonas axonopodis pv malvacearum and cotton leaf curl virus
    Chakrabarty, PK
    Sable, S
    Monga, D
    Mayee, CD
    INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 2005, 75 (08): : 524 - 528
  • [10] POLYMERASE CHAIN REACTION-BASED METHODS FOR THE DETECTION OF MUTATIONS IN ONCOGENES AND TUMOR-SUPPRESSOR GENES
    LODA, M
    HUMAN PATHOLOGY, 1994, 25 (06) : 564 - 571
12345