Iron-binding characterization and polysaccharide production by Klebsiella oxytoca strain isolated from mine acid drainage

被引：0
|
作者：
Baldi, F. [1 ]
Marchetto, D. [1 ]
Battistel, D. [2 ]
Daniele, S. [2 ]
Faleri, C. [3 ]
De Castro, C. [4 ]
Lanzetta, R. [4 ]
机构：
[1] Department of Environmental Science, Cà Foscari University of Venezia, Calle Larga S. Marta, 30121 Venezia, Italy
[2] Department of Physical Chemistry, Cà Foscari University of Venezia, Venezia, Italy
[3] Department of Environmental Science 'G. Sarfatti', University of Siena, Siena, Italy
[4] Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Naples, Italy
来源：
Journal of Applied Microbiology | 2009年 / 107卷 / 04期
关键词：
Aims: To investigate Klebsiella oxytoca strain BAS-10 growth on ferric citrate under anaerobic conditions for exopolysaccharide (EPS) production and localization on cell followed by the purification and the EPS determination of the iron-binding stability constant to EPS or biotechnological applications. Methods and Results: Klebsiella oxytoca ferments ferric citrate under anaerobic conditions and produces a ferric hydrogel; whereas ferrous ions were formed in solution. During growth; cells precipitate and a hydrogel formation was observed: the organic material was constituted of an EPS bound to Fe(III) ions; this was found by chemical analyses of the iron species and transmission electron microscopy of the cell cultures. Iron binding to EPS was studied by cyclic voltammetric measurements; either directly on the hydrogel or in an aqueous solutions containing Fe(III)-citrate and purified Fe(III)-EPS. From the voltammetric data; the stability constant for the Fe(III)-EPS complex can be assumed to have values of approx. 1012-1013. It was estimated that this is higher than for the Fe(III)-citrate complex. Conclusions: The production of Fe(III)-EPS under anaerobic conditions is a strategy for the strain to survive in mine drainages and other acidic conditions. This physiological feature can be used to produce large amounts of valuable Fe(III)-EPS; starting from a low cost substrate such as Fe(III)-citrate. Significant and Impact of the Study: The data herein demonstrates that an interesting metal-binding molecule can be produced as a novel catalyst for a variety of potential applications and the EPS itself is a valuable source for rhamnose purification. © 2009 The Society for Applied Microbiology;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：1241 / 1250
相关论文
共 50 条
  • [1] Iron-binding characterization and polysaccharide production by Klebsiella oxytoca strain isolated from mine acid drainage
    Baldi, F.
    Marchetto, D.
    Battistel, D.
    Daniele, S.
    Faleri, C.
    De Castro, C.
    Lanzetta, R.
    JOURNAL OF APPLIED MICROBIOLOGY, 2009, 107 (04) : 1241 - 1250
  • [2] XAS analysis of a nanostructured iron polysaccharide produced anaerobically by a strain of Klebsiella oxytoca
    Iztok Arčon
    Oreste Piccolo
    Stefano Paganelli
    Franco Baldi
    BioMetals, 2012, 25 : 875 - 881
  • [3] XAS analysis of a nanostructured iron polysaccharide produced anaerobically by a strain of Klebsiella oxytoca
    Arcon, Iztok
    Piccolo, Oreste
    Paganelli, Stefano
    Baldi, Franco
    BIOMETALS, 2012, 25 (05) : 875 - 881
  • [4] Isolation and partial characterization of a polysaccharide produced by Klebsiella oxytoca ICCF 419, a newly-isolated strain in Romania
    Tomulescu, Caterina
    Moscovici, Misu
    Stoica, Roxana Madalina
    Casarica, Angela
    Vladu, Mariana
    Nita, Sultana
    Donciu, Roxana
    Bejanaru, Ionica
    Neagu, Georgeta
    Ionescu, Robertina
    Hanganu, Anamaria
    Stanciu, Cristina
    Bazdoaca, Cristina
    Vamanu, Adrian
    ROMANIAN BIOTECHNOLOGICAL LETTERS, 2020, 25 (05): : 1861 - 1876
  • [5] XAS analysis of iron and palladium bonded to a polysaccharide produced anaerobically by a strain of Klebsiella oxytoca
    Arcon, Iztok
    Paganelli, Stefano
    Piccolo, Oreste
    Gallo, Michele
    Vogel-Mikus, Katarina
    Baldi, Franco
    JOURNAL OF SYNCHROTRON RADIATION, 2015, 22 : 1215 - 1226
  • [6] Adsorption performance investigation of heavy metal copper in acid mine drainage by the Klebsiella oxytoca
    Zhu, Y. J.
    Zhou, L. B.
    Zhu, Y. B.
    Ju, L. P.
    LEGISLATION, TECHNOLOGY AND PRACTICE OF MINE LAND RECLAMATION, 2015, : 397 - 402
  • [7] Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages
    Gallo, Giuseppe
    Presta, Luana
    Perrin, Elena
    Gallo, Michele
    Marchetto, Davide
    Puglia, Anna Maria
    Fani, Renato
    Baldi, Franco
    BMC MICROBIOLOGY, 2018, 18
  • [8] Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages
    Giuseppe Gallo
    Luana Presta
    Elena Perrin
    Michele Gallo
    Davide Marchetto
    Anna Maria Puglia
    Renato Fani
    Franco Baldi
    BMC Microbiology, 18
  • [9] Total synthesis of the heptasaccharide repeating unit of the iron-binding exopolysaccharide secreted by Klebsiella oxytoca BAS-10
    Guchhait, Goutam
    Misra, Anup Kumar
    TETRAHEDRON-ASYMMETRY, 2009, 20 (15) : 1791 - 1797
  • [10] Production of Iron Pigments (Goethite and Haematite) from Acid Mine Drainage
    Silva, Rodrigo de Almeida
    Castro, Carmen Dias
    Petter, Carlos Otavio
    Homrich Schneider, Ivo Andre
    MINE WATER - MANAGING THE CHALLENGES: PROCEEDINGS OF THE INTERNATIONAL MINE WATER ASSOCIATION CONGRESS 2011, 2011, : 469 - 473
12345