Observation of pronounced b&bull,y cleavages in the electron capture dissociation mass spectrometry of polyamidoamine (PAMAM) dendrimer ions with amide functionalities

被引：0

作者：

Lee, Sunyoung ^{[1
]}

Han, Sang Yun ^{[2
]}

Lee, Tae Geol ^{[2
]}

Chung, Gyusung ^{[3
]}

Lee, Duckhwan ^{[1
]}

Oh, Han Bin ^{[1
,4
]}

机构：

[1] Department of Chemistry, Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, Korea, Republic of

[2] Division of Chemical Metrology and Materials Evaluation, Korea Research Institute of Standards and Science, Daejeon, Korea, Republic of

[3] Department of Chemistry, Konyang University, Chungnam, Korea, Republic of

[4] Department of Chemistry, Interdisciplinary Program of Integrated Biotechnology, Sogang University, Sinsudong 1, Mapogu, Seoul 121-742, Korea, Republic of

来源：

Journal of the American Society for Mass Spectrometry | 2006年 / 17卷 / 04期

关键词：

We report the electron capture dissociation (ECD) mass spectrometry of the third generation polyamidoamine (PAMAM) dendrimer that contains amide functionalities. The dendrimer was chosen because it offers a unique opportunity to understand the ECD behavior of the amide functionality in a framework other than peptides/proteins. In this study; PAMAM ECD was found to exhibit a fragmentation pattern strikingly different from that of ordinary peptide/protein ECD. Specifically; ECD of multiply protonated PAMAM ions gave rise to significant b• y cleavages as well as S; E dissociations but; unexpectedly; only minor c; z&bull fragmentations are observed. In an effort to account for the unexpectedly different fragmentation pattern; a comparative ECD experiment on the poly(propylene imine) dendrimer in which the amide bond moiety is not available and density functional theory calculations (B3LYP/6-311 + G(d)) investigations on the model system of a charge-solvated single-repeat unit were carried out. On the basis of these results; we discuss here possible implications of intramolecular charge-solvation; energy barriers in dissociation reactions; and macromolecular properties of the dendritic molecule for understanding the reaction pathway of PAMAM ECD. © 2006 American Society for Mass Spectrometry;

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摘要：

Journal article (JA)

引用

页码：536 / 543

共 5 条

[1] Observation of pronounced b•,y cleavages in the electron capture dissociation mass spectrometry of polyamidoamine (PAMAM) dendrimer ions with amide functionalities
Lee, S
Han, SY
Lee, TG
Chung, G
Lee, D
Bin Oh, H
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2006, 17 (04) : 536 - 543
[2] Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups
Kaczorowska, Malgorzata A.
Cooper, Helen J.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (09) : 1312 - 1319
[3] Electron Capture Dissociation, Electron Detachment Dissociation, and Collision-Induced Dissociation of Polyamidoamine (PAMAM) Dendrimer Ions with Amino, Amidoethanol, and Sodium Carboxylate Surface Groups
Kaczorowska, Malgorzata A.
Cooper, Helen J.
Journal of the American Society for Mass Spectrometry, 2008, 19 (09): : 1312 - 1319
[4] Electron capture dissociation mass spectrometry of peptide cations containing a lysine homologue: a mobile proton model for explaining the observation of b-type product ions
Lee, Sunyoung
Chung, Gyusung
Kim, Jaedong
Oh, Han Bin
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (21) : 3167 - 3175
[5] Comparable electron capture efficiencies for various protonated sites on the 3rd generation poly(propylene imine) dendrimer ions:: Applications by SORI-CAD and electron capture dissociation mass spectrometry (ECD MS)
Han, SY
Lee, S
Oh, HB
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2005, 26 (05) : 740 - 746

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