A high affinity and selective DNA aptamer for copper ions

被引：2
|
作者：
Wang, Jin [1 ,2 ]
Liu, Yibo [2 ]
Li, Xiangmei [1 ]
Lei, Hongtao [1 ]
Liu, Juewen [2 ]
机构：
[1] South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China
[2] Univ Waterloo, Waterloo Inst Nanotechnol, Dept Chem, Waterloo, ON N2L 3G1, Canada
来源：
CHEMICAL COMMUNICATIONS | 2024年 / 60卷 / 96期
基金：
加拿大自然科学与工程研究理事会;
关键词：
LIGHT-UP BIOSENSOR; AQUEOUS-SOLUTION; ICP-MS; DNAZYME; CU2+; BEACON; PROBE; ZN; PB; CD;
D O I：
10.1039/d4cc05410e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Capture-SELEX was employed for the selection of DNA aptamers for Cu2+. The best aptamer named Cu-1 has a Kd of 14.2 nM as determined using the strand-displacement assay, representing an approximate 3000-fold improvement over a previously reported Cu2+ aptamer. The sensor achieved a limit of detection of 2.1 nM.
引用
收藏
页码：14272 / 14275
页数：4
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